Rabbit anti-Mouse, Rat Batf2 Polyclonal Antibody | anti-BATF2 antibody

Anti-Batf2 Antibody Picoband

Cat. Num. AAA126564

• Gene Names: Batf2; 4933430F08Rik
• Reactivity: Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: Batf2, Antibody; Anti-Batf2 Antibody Picoband; anti-BATF2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-BATF2 antibody: ELISA, IHC (Immunohistochemistry), WB (Western Blot)
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived mouse Batf2 recombinant protein (Position: M1-L138).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

IHC (Immunohistochemisry)

(Figure 3. IHC analysis of Batf2 using anti-Batf2 antibody (AAA126564).Batf2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Batf2 Antibody (AAA126564) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry)

(Figure 2. IHC analysis of Batf2 using anti-Batf2 antibody (AAA126564).Batf2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Batf2 Antibody (AAA126564) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of Batf2 using anti-Batf2 antibody (AAA126564).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat C6 whole cell lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Batf2 antigen affinity purified polyclonal antibody (#AAA126564) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Batf2 at approximately 25 kDa. The expected band size for Batf2 is at 25 kDa.)

Related Product Information for anti-BATF2 antibody

Basic leucine zipper transcription factor, ATF-like 2 is a protein that, in humans, is encoded by the BATF2 gene. BATF2 (basic leucine zipper transcription factor, ATF-like 2) is a 274 amino acid protein that localizes to the nucleus and contains one bZIP domain, suggesting that it may be involved in transcriptional regulation. The gene encoding BATF2, which is expressed as multiple alternatively spliced isoforms, is located on human chromosome 11. With approximately 135 million base pairs and 1,400 genes, chromosome 11 comprises approximately 4% of human genomic DNA and is considered a gene and disease association dense chromosome. The chromosome 11 encoded Atm gene is important for regulation of cell cycle arrest and apoptosis following double strand DNA breaks. Atm mutation leads to the disorder known as ataxia-telangiectasia. The blood disorders Sickle cell anemia and thalassemia are caused by HBB gene mutations, while Wilms' tumors, WAGR syndromeand Denys-Drash syndrome are associated with mutations of the WT1 gene. Jervell and Lange-Nielsen syndrome,Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-LemliOpitz syndrome are alsoassociated with defects in chromosome 11-encoded genes.

Product Categories/Family for anti-BATF2 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 28077033
• NCBI GeneID: 74481
• NCBI Accession #: NP_083243.1
• NCBI GenBank Nucleotide #: NM_028967.1
• UniProt Accession #: Q8R1H8; Q3TAI2
• Molecular Weight: 29,703 Da
• NCBI Official Full Name: basic leucine zipper transcriptional factor ATF-like 2
• NCBI Official Synonym Full Names: basic leucine zipper transcription factor, ATF-like 2
• NCBI Official Symbol: Batf2
• NCBI Official Synonym Symbols: 4933430F08Rik
• NCBI Protein Information: basic leucine zipper transcriptional factor ATF-like 2; B-ATF-2
• UniProt Protein Name: Basic leucine zipper transcriptional factor ATF-like 2
• UniProt Gene Name: Batf2
• UniProt Synonym Gene Names: B-ATF-2
• UniProt Entry Name: BATF2_MOUSE