IHC (Immunohiostchemistry)
(BBC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BBC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human BBC3 Polyclonal Antibody | anti-BBC3 antibody
BBC3 Antibody (Center)
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BBC3, Antibody; BBC3 Antibody (Center); Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis; BBC3; PUMA; anti-BBC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BBC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the Central region of human BBC3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
109-137
Sequence Length
261
Applicable Applications for anti-BBC3 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(BBC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BBC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(BBC3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BBC3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(BBC3 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BBC3 antibody detected the BBC3 protein (arrow).)
WB (Western Blot)
(BBC3 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BBC3 antibody detected the BBC3 protein (arrow).)
Related Product Information for anti-BBC3 antibody
BBC3 is an essential mediator of p53-dependent and p53-independent apoptosis.
Product Categories/Family for anti-BBC3 antibody
References
Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) :
Gurzov, E.N., et al. J. Biol. Chem. 285(26):19910-19920(2010)
Hemmati, P.G., et al. J. Mol. Med. 88(6):609-622(2010)
Gomes, N.P., et al. Genes Dev. 24(10):1022-1034(2010)
Liu, F.T., et al. Biochem. Biophys. Res. Commun. 310(3):956-962(2003)
Gurzov, E.N., et al. J. Biol. Chem. 285(26):19910-19920(2010)
Hemmati, P.G., et al. J. Mol. Med. 88(6):609-622(2010)
Gomes, N.P., et al. Genes Dev. 24(10):1022-1034(2010)
Liu, F.T., et al. Biochem. Biophys. Res. Commun. 310(3):956-962(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20532
NCBI Official Full Name
bcl-2-binding component 3 isoform 1
NCBI Official Synonym Full Names
BCL2 binding component 3
NCBI Official Symbol
BBC3
NCBI Official Synonym Symbols
JFY1; PUMA; JFY-1
NCBI Protein Information
bcl-2-binding component 3
UniProt Protein Name
Bcl-2-binding component 3
UniProt Gene Name
BBC3
UniProt Synonym Gene Names
PUMA
UniProt Entry Name
BBC3B_HUMAN
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Product Notes
The BBC3 bbc3 (Catalog #AAA289328) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 109-137. The BBC3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BBC3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BBC3 bbc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BBC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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