WB (Western Blot)
(BCL2 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the BCL2 antibody detected the BCL2 protein (arrow).)
Rabbit BCL2 Polyclonal Antibody | anti-BCL2 antibody
BCL2 Antibody (Center)
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
HumanPredicted Reactivity: Bovine, Chicken, Hamster, Mouse, Rat
Applications
ELISA, Western Blot
Synonyms
BCL2, Antibody; BCL2 Antibody (Center); Apoptosis regulator Bcl-2; BCL2; anti-BCL2 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Bovine, Chicken, Hamster, Mouse, Rat
Predicted Reactivity: Bovine, Chicken, Hamster, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-171 amino acids from the Central region of human BCL2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
144-171
Sequence Length
239
Applicable Applications for anti-BCL2 antibody
ELISA, WB (Western Blot)
Clone Names
RB41153
Antigen Source
HUMAN
Function
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed: ).
Cellular Location
Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location
Expressed in a variety of tissues.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(BCL2 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the BCL2 antibody detected the BCL2 protein (arrow).)
WB (Western Blot)
(BCL2 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the BCL2 antibody detected the BCL2 protein (arrow).)
Related Product Information for anti-BCL2 antibody
This gene encodes an integral outer mitochondrial membrane
protein that blocks the apoptotic death of some cells such as
lymphocytes. Constitutive expression of BCL2, such as in the case
of translocation of BCL2 to Ig heavy chain locus, is thought to be
the cause of follicular lymphoma. Two transcript variants, produced
by alternate splicing, differ in their C-terminal ends. [provided
by RefSeq].
protein that blocks the apoptotic death of some cells such as
lymphocytes. Constitutive expression of BCL2, such as in the case
of translocation of BCL2 to Ig heavy chain locus, is thought to be
the cause of follicular lymphoma. Two transcript variants, produced
by alternate splicing, differ in their C-terminal ends. [provided
by RefSeq].
Product Categories/Family for anti-BCL2 antibody
References
Feng, H., et al. Cancer Cell 18(4):353-366(2010)
Azad, N., et al. Ann. N. Y. Acad. Sci. 1203, 1-6 (2010) :
Dubikov, A.I., et al. Scand. J. Rheumatol. 39(5):368-372(2010)
Yu, B., et al. J. Exp. Clin. Cancer Res. 29, 107 (2010) :
Trisciuoglio, D., et al. PLoS ONE 5 (7), E11772 (2010) :
Azad, N., et al. Ann. N. Y. Acad. Sci. 1203, 1-6 (2010) :
Dubikov, A.I., et al. Scand. J. Rheumatol. 39(5):368-372(2010)
Yu, B., et al. J. Exp. Clin. Cancer Res. 29, 107 (2010) :
Trisciuoglio, D., et al. PLoS ONE 5 (7), E11772 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 26266
NCBI Official Full Name
apoptosis regulator Bcl-2 alpha isoform
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 2
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
NCBI Protein Information
apoptosis regulator Bcl-2
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
UniProt Entry Name
BCL2_HUMAN
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Product Notes
The BCL2 bcl2 (Catalog #AAA286509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 144-171. The BCL2 Antibody (Center) reacts with Human Predicted Reactivity: Bovine, Chicken, Hamster, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BCL2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BCL2 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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