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IHC (Immunohistochemistry)
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat BDNF Polyclonal Antibody | anti-BDNF antibody
Goat anti-BDNF Antibody
Gene Names
BDNF; ANON2; BULN2
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Immunofluorescence, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BDNF, Antibody; Goat anti-BDNF Antibody; BDNF, brain-derived neurotrophic factor, MGC34632, neurotrophin; anti-BDNF antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Applicable Applications for anti-BDNF antibody
IF (Immunofluorescence), ELISA (Peptide ELISA)
Important Comments
This antibody is expected to recognize all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1)
Immunogen
Peptide with sequence ETKCNPMGYTKE, from the internal region of the protein sequence according to NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IHC (Immunohistochemistry)
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
(AAA61777 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex. Antigen retrieval with citrate buffer pH 6 at 95-98C, DAB-staining. This data is from a previous batch, not on sale.)
IHC (Immunohistochemistry)
(AAA61777 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex. Antigen retrieval with citrate buffer pH 6 at 95-98C, DAB-staining. This data is from a previous batch, not on sale.)
IHC (Immunohistochemisry)
(AAA61777 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C. This data is from a previous batch, not on sale.)
IHC (Immunohistochemisry)
(AAA61777 (5ug/ml) staining of paraffin embedded Rat Cerebral Cortex (A) and Rat Hippocampus (B), CRY-staining with DAPI nuclear counter stain in blue. Antigen retrieval with citrate buffer pH 6 at 95-98C. This data is from a previous batch, not on sale.)
IF (Immunofluorescence)
(AAA61777 (1ug/ml) staining of PFA-perfused cryosection of Mouse sciatic nerve (left) and injured sciatic nerve (right). Alexa 488-staining. Data obtained from Simon Glerup, Department of Biomedicine, Aarhus University, Denmark.This data is from a previous batch, not on sale.)
IF (Immunofluorescence)
(AAA61777 (1ug/ml) staining of PFA-perfused cryosection of Mouse sciatic nerve (left) and injured sciatic nerve (right). Alexa 488-staining. Data obtained from Simon Glerup, Department of Biomedicine, Aarhus University, Denmark.This data is from a previous batch, not on sale.)
IF (Immunofluorescence)
(AAA61777 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA61777 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
brain-derived neurotrophic factor isoform a preproprotein
NCBI Official Synonym Full Names
brain-derived neurotrophic factor
NCBI Official Symbol
BDNF
NCBI Official Synonym Symbols
ANON2; BULN2
NCBI Protein Information
brain-derived neurotrophic factor
UniProt Protein Name
Brain-derived neurotrophic factor
UniProt Gene Name
BDNF
UniProt Synonym Gene Names
BDNF
UniProt Entry Name
BDNF_HUMAN
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Product Notes
The BDNF bdnf (Catalog #AAA61777) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BDNF Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's BDNF can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the BDNF bdnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BDNF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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