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IP (Immunoprecipitation)
(B2M was immunoprecipitated using:; Lane A:0.5 mg A-431 Whole Cell Lysate; Lane B:0.5 mg HeLa Whole Cell Lysate; Lane C:0.5 mg HL-60 Whole Cell Lysate; 4 uL anti- B2M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti- B2M rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 14kDa; Observed band size :12 kDa)
Rabbit anti-Human Beta-2 microglobulin Polyclonal Antibody | anti-B2M antibody
Beta-2 microglobulin Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Synonyms
Beta-2 microglobulin, Antibody; Beta-2 microglobulin Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-Beta-2 microglobulin Antibody; anti-B2M antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human Beta-2 microglobulin
Applicable Applications for anti-B2M antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Preparation
Produced in rabbits immunized with purified, recombinant Human Beta-2 microglobulin (rh Beta-2 microglobulin;NP_004039.1; Met1-Met119). Beta-2 microglobulin specific IgG was purified by Human Beta-2 microglobulin affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(B2M was immunoprecipitated using:; Lane A:0.5 mg A-431 Whole Cell Lysate; Lane B:0.5 mg HeLa Whole Cell Lysate; Lane C:0.5 mg HL-60 Whole Cell Lysate; 4 uL anti- B2M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti- B2M rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 14kDa; Observed band size :12 kDa)
IP (Immunoprecipitation)
(B2M was immunoprecipitated using:; Lane A:0.5 mg A-431 Whole Cell Lysate; Lane B:0.5 mg HeLa Whole Cell Lysate; Lane C:0.5 mg HL-60 Whole Cell Lysate; 4 uL anti- B2M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti- B2M rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 14kDa; Observed band size :12 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human Beta-2 microglobulin in human ovarian cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human Beta-2 microglobulin in human ovarian cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Beta-2 microglobulin in human renal carcinoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human Beta-2 microglobulin in human renal carcinoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-Beta-2 microglobulin rabbit polyclonal antibody at 1:500 dilution; Lane A: A-431 Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lane C: HL60 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:14 kDa; Observed band size:12 kDa)
WB (Western Blot)
(Anti-Beta-2 microglobulin rabbit polyclonal antibody at 1:500 dilution; Lane A: A-431 Whole Cell Lysate; Lane B: HeLa Whole Cell Lysate; Lane C: HL60 Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:14 kDa; Observed band size:12 kDa)
Related Product Information for anti-B2M antibody
B2M, also known as beta2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (beta2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the alpha3 chain. Unlike alpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M (beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (beta2 microglobulin) can indicate non-progression of HIV.
Product Categories/Family for anti-B2M antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Protein Information
beta-2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin
UniProt Protein Name
Beta-2-microglobulin
UniProt Gene Name
B2M
UniProt Entry Name
B2MG_HUMAN
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Product Notes
The B2M b2m (Catalog #AAA258634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Beta-2 microglobulin Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-2 microglobulin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the B2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-2 microglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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