ICC (Immunocytochemistry)
(Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 ug/mL.)
Rabbit Beta-actin Polyclonal Antibody | anti-ACTB antibody
Beta-actin Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column
Synonyms
Beta-actin, Antibody; Beta-actin Antibody; Beta actin, b actin; anti-ACTB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
375
Applicable Applications for anti-ACTB antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Raised against a 16 amino acid peptide from near the amino-terminus of human b-actin.
Target Name
Beta-actin
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ICC (Immunocytochemistry)
(Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 ug/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 ug/mL.)
WB (Western Blot)
(Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)
WB (Western Blot)
(Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)
Related Product Information for anti-ACTB antibody
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Product Categories/Family for anti-ACTB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
41,737 Da
NCBI Official Full Name
Actin, beta
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
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Product Notes
The ACTB actb (Catalog #AAA303389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Beta-actin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-actin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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