<
IHC (Immunohistochemistry)
(IHC of human mouse heart using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
beta-Actin Polyclonal Antibody | anti-beta-actin antibody
Anti-beta-Actin
Reactivity
Human, Rat, Mouse, Monkey, and Canine proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Synonyms
beta-Actin, Antibody; Anti-beta-Actin; ACTB; beta-actin; BRWS1; PS1TP5BP1 antibody; anti-beta-actin antibody
Reactivity
Human, Rat, Mouse, Monkey, and Canine proteins
Clonality
Polyclonal
Specificity
Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
Form/Format
Polyclonal antibody supplied as a 200 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Sequence Length
124
Applicable Applications for anti-beta-actin antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry Frozen (IHC-F), Immunohistochemistry Paraffin (IHC-P)
Application Notes
Western Blot (WB): 1:250-1:2,000
Immunofluorescence (IF): 1:50-1:250
Immunohistochemistry Frozen (IHC-F): 1:250 1:1000
Immunohistochemistry Paraffin (IHC-P): 1:250 1:1000
Immunofluorescence (IF): 1:50-1:250
Immunohistochemistry Frozen (IHC-F): 1:250 1:1000
Immunohistochemistry Paraffin (IHC-P): 1:250 1:1000
Source
Goat
Immunogen
Recombinant peptide derived form within residues 100 aa to the N-terminus of human beta-Actin produced in E.coli.
Note
The antibody solution should be gently mixed before use
Preparation and Storage
Store at 2-8°C for one-two days.
For extended storage, Store at -20°C
Working dilution samples should discarded if not used within 12 hours
For extended storage, Store at -20°C
Working dilution samples should discarded if not used within 12 hours
IHC (Immunohistochemistry)
(IHC of human mouse heart using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human mouse heart using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IHC (Immunohistchemistry)
(IHC of human appendix using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IHC (Immunohistchemistry)
(IHC of human appendix using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human lymph node using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection)
IHC (Immunohistochemistry)
(IHC of human lymph node using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection)
IF (Immunofluorescence)
(Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin)
IF (Immunofluorescence)
(Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin)
IHC (Immunohistchemistry)
(IHC of mouse liver using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IHC (Immunohistchemistry)
(IHC of mouse liver using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IF (Immunofluorescence)
(Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin)
IF (Immunofluorescence)
(Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin)
IHC (Immunohistochemistry)
(IHC of mouse kidney using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IHC (Immunohistochemistry)
(IHC of mouse kidney using anti-b-Actin antibody and FFPE tissue after heat-induced antigen retrieval. Anti-b-Actin Ab at 1:500/DAB detection.)
IF (Immunofluorescence)
(Anti-beta Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin.)
IF (Immunofluorescence)
(Anti-beta Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin.)
Application Data
(Anti-beta-Actin Ab at 1/500 dilution; lysates at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution)
Application Data
(Anti-beta-Actin Ab at 1/500 dilution; lysates at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution)
Application Data
Application Data
Related Product Information for anti-beta-actin antibody
Actin proteins are highly conserved and ubiquitously expressed in all eukaryotic cells. These proteins are the major components of the cytoskeleton and involved in various types of cell motility.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
beta-actin, partial
UniProt Protein Name
Beta-actin
UniProt Gene Name
beta-actin
UniProt Entry Name
K4NQ23_9EURO
Similar Products
Product Notes
The beta-Actin beta-actin (Catalog #AAA13875) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-beta-Actin reacts with Human, Rat, Mouse, Monkey, and Canine proteins and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry Frozen (IHC-F), Immunohistochemistry Paraffin (IHC-P). Western Blot (WB): 1:250-1:2,000 Immunofluorescence (IF): 1:50-1:250 Immunohistochemistry Frozen (IHC-F): 1:250 1:1000 Immunohistochemistry Paraffin (IHC-P): 1:250 1:1000. Researchers should empirically determine the suitability of the beta-Actin beta-actin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.