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product-image-AAA13863_IHC10.jpg IHC (Immunohistochemistry) (IHC of mouse retina using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

Goat beta-Catenin Polyclonal Antibody | anti-beta-Catenin antibody

Catenin beta 1 Polyclonal Antibody

Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human, rat, mouse, canine, and monkey proteins.
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
beta-Catenin, Antibody; Catenin beta 1 Polyclonal Antibody; Anti-beta-Catenin; cadherin-associated protein; CTNNB; CTNNB1; MRD19; armadillo antibody; anti-beta-Catenin antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human, rat, mouse, canine, and monkey proteins.
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects a band of approximately 95 kDa by Western blot in MNT1 and SH-SY5Y cell lysates.
Form/Format
Polyclonal antibody supplied as a 200 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Sequence Length
781
Applicable Applications for anti-beta-Catenin antibody
Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2,000
IF: 1:50-1:250
IHC-P 1:250-1:1000
Immunogen
Purified recombinant peptide derived from within residues 731 aa to the C-terminus of human beta Catenin produced in E. coli.
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours Avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(IHC of mouse retina using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

product-image-AAA13863_IHC10.jpg IHC (Immunohistochemistry) (IHC of mouse retina using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

IF (Immunofluorescence)

(Confocal immunofluorescence – anti-Catenin beta1 Ab in BEAS-2B cells at 1/250 dilution; cells were fixed with 4% of PFA;)

product-image-AAA13863_IF8.jpg IF (Immunofluorescence) (Confocal immunofluorescence – anti-Catenin beta1 Ab in BEAS-2B cells at 1/250 dilution; cells were fixed with 4% of PFA;)

IHC (Immunohistochemistry)

(IHC of mouse lung using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

product-image-AAA13863_IHC7.jpg IHC (Immunohistochemistry) (IHC of mouse lung using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

IHC (Immunohistchemistry)

(IHC of mouse brain using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

product-image-AAA13863_IHC6.jpg IHC (Immunohistchemistry) (IHC of mouse brain using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

IHC (Immunohistochemistry)

(IHC of mouse liver using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

product-image-AAA13863_IHC5.jpg IHC (Immunohistochemistry) (IHC of mouse liver using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

IHC (Immunohistochemistry)

(IHC of mouse intestine using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

product-image-AAA13863_IHC4.jpg IHC (Immunohistochemistry) (IHC of mouse intestine using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 Ab at 1:500/DAB detection.)

IF (Immunofluorescence)

(Confocal immunofluorescence – anti-Catenin beta1 Ab in BEAS-2B cells at 1/250 dilution; cells were fixed with 4% of PFA;)

product-image-AAA13863_IF3.jpg IF (Immunofluorescence) (Confocal immunofluorescence – anti-Catenin beta1 Ab in BEAS-2B cells at 1/250 dilution; cells were fixed with 4% of PFA;)

WB (Western Blot)

(Anti-beta-Catenin Ab at 1/1,000 dilution; lysate at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)

product-image-AAA13863_WB2.jpg WB (Western Blot) (Anti-beta-Catenin Ab at 1/1,000 dilution; lysate at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)

Reactivity Chart

product-image-AAA13863_REACTIVITY.jpg Reactivity Chart
Related Product Information for anti-beta-Catenin antibody
Catenin (cadherin-associated protein), beta 1, 88kDa

Goat polyclonal antibody to Catenin (cadherin-associated protein), beta 1. Beta Catenin is a 88 kDa protein and is a key downstream effector in the Wnt signalling pathway. It is involved in early embryonic development and tumorigenesis. Beta Catenin is part of a complex of proteins that constitute adherens junctions, necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. This protein anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
beta-catenin
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol
CTNNB1
NCBI Official Synonym Symbols
CTNNB; MRD19; armadillo
NCBI Protein Information
catenin beta-1
UniProt Protein Name
Catenin beta-1
UniProt Gene Name
CTNNB1
UniProt Synonym Gene Names
CTNNB
UniProt Entry Name
CTNB1_HUMAN

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Product Notes

The beta-Catenin ctnnb1 (Catalog #AAA13863) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Catenin beta 1 Polyclonal Antibody reacts with Human, rat, mouse, canine, and monkey proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Catenin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry Paraffin (IHC-P). WB: 1:500-1:2,000 IF: 1:50-1:250 IHC-P 1:250-1:1000. Researchers should empirically determine the suitability of the beta-Catenin ctnnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Catenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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