WB (Western Blot)
(All lanes : Anti-beta Tubulin Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C2C12 whole cell lysateLane 7: PC-12 whole cell lysateLane 8: C6 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit beta Tubulin Polyclonal Antibody | anti-Tubb5 antibody
beta Tubulin
Gene Names
Tubb5; AA408537; AI596182; M(beta)5; B130022C14Rik
Reactivity
Human, Mouse, RatPredicted: C.Elegans, Chicken, Drosophila, Monkey, Bovine, Xenopus, Hamster, Pig
Applications
ELISA, Western Blot
Synonyms
beta Tubulin, Antibody; beta Tubulin; Tubulin beta-5 chain; Tubb5; anti-Tubb5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: C.Elegans, Chicken, Drosophila, Monkey, Bovine, Xenopus, Hamster, Pig
Predicted: C.Elegans, Chicken, Drosophila, Monkey, Bovine, Xenopus, Hamster, Pig
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human.
Sequence Length
444
Applicable Applications for anti-Tubb5 antibody
ELISA, WB (Western Blot)
Source
Human
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Cellular Location
Cytoplasm, cytoskeleton
Tissue Location
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Expressed in embryonic brain, including throughout the developing cortex and in the subventricular zone. Also found in radial glial cells, intermediate progenitors, migrating neurons and postmitotic neurons (PubMed:23246003). Expressed in skin and developing hair follicle (PubMed:26637975)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-beta Tubulin Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C2C12 whole cell lysateLane 7: PC-12 whole cell lysateLane 8: C6 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-beta Tubulin Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C2C12 whole cell lysateLane 7: PC-12 whole cell lysateLane 8: C6 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 55 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing NIH/3T3 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing NIH/3T3 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Related Product Information for anti-Tubb5 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Product Categories/Family for anti-Tubb5 antibody
References
Wang D.,et al.J. Cell Biol. 103:1903-1910(1986). Carninci P.,et al.Science 309:1559-1563(2005). Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009). Lubec G.,et al.Submitted (JUL-2007) to UniProtKB. Lewis S.A.,et al.J. Cell Biol. 101:852-861(1985).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
M=50 kda
NCBI Official Full Name
tubulin beta-5 chain
NCBI Official Synonym Full Names
tubulin, beta 5 class I
NCBI Official Symbol
Tubb5
NCBI Official Synonym Symbols
AA408537; AI596182; M(beta)5; B130022C14Rik
NCBI Protein Information
tubulin beta-5 chain
UniProt Protein Name
Tubulin beta-5 chain
UniProt Gene Name
Tubb5
Similar Products
Product Notes
The Tubb5 tubb5 (Catalog #AAA290957) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta Tubulin reacts with Human, Mouse, Rat Predicted: C.Elegans, Chicken, Drosophila, Monkey, Bovine, Xenopus, Hamster, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's beta Tubulin can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Tubb5 tubb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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