Rabbit BID Polyclonal Antibody | anti-BID antibody

BID Antibody

Cat. Num. AAA29655

• Gene Names: BID; FP497
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: BID; Polyclonal Antibody; BID Antibody; FP497; MGC15319; MGC42355; anti-BID antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total BID protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 195

• Applicable Applications for anti-BID antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human BID.
• Target Name: BID
• Preparation and Storage: Store at -20 degree C

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded human stomach cancer using Bid antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using BID antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human lung cancer using BID antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat brain using BID antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Colon cancer using BID antibody.)

WB (Western Blot)

(Western blot analysis of extracts of HL60 cell lines, using BID antibody.)

Related Product Information for anti-BID antibody

The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.

Product Categories/Family for anti-BID antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4557361
• NCBI GeneID: 637
• NCBI Accession #: NP_001187.1
• NCBI GenBank Nucleotide #: NM_001196.3
• UniProt Accession #: P55957; Q549M7; Q71T04; Q7Z4M9; Q8IY86
• Molecular Weight: 11,263 Da
• NCBI Official Full Name: BH3-interacting domain death agonist isoform 2
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: BID
• NCBI Official Synonym Symbols: FP497
• NCBI Protein Information: BH3-interacting domain death agonist
• UniProt Protein Name: BH3-interacting domain death agonist
• UniProt Gene Name: BID
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_HUMAN