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IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody. Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilution of 1:1000.)
Rabbit anti-Human, Mouse CDK4 Polyclonal Antibody | anti-CDK4 antibody
[KO Validated] CDK4 Rabbit pAb
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Synonyms
CDK4; Polyclonal Antibody; [KO Validated] CDK4 Rabbit pAb; CMM3; PSK-J3; cyclin-dependent kinase 4; anti-CDK4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Applicable Applications for anti-CDK4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IP: 1:50-1:200
IF/ICC: 1:50-1:200
IHC: 1:50-1:100
IP: 1:50-1:200
IF/ICC: 1:50-1:200
Positive Samples
HeLa, 293T
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-303 of human CDK4 (NP_000066.1).
Cellular Location
Cytoplasm, Membrane, Nucleus
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody. Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody. Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilution of 1:1000.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 using CDK4 antibody at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 using CDK4 antibody at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon using CDK4 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon using CDK4 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of extracts from wild type (WT) and CDK4 knockout (KO) HeLa cells, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts from wild type (WT) and CDK4 knockout (KO) HeLa cells, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of 293T cells, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-CDK4 antibody
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Product Categories/Family for anti-CDK4 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CDK4 (Catalog #AAA28411) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KO Validated] CDK4 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CDK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IHC: 1:50-1:100 IP: 1:50-1:200 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CDK4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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