Rabbit anti-Mouse BID Polyclonal Antibody | anti-BID antibody

Anti-BID Antibody, Rabbit Polyclonal

Cat. Num. AAA257139

• Gene Names: Bid; AI875481; AU022477; 2700049M22Rik
• Reactivity: Mouse
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: BID, Antibody; Anti-BID Antibody, Rabbit Polyclonal; p22 BID Antibody, Rabbit PAb, Antigen Affinity Purified; BH3 interacting domain death agonist; Anti-2700049M22Rik Antibody; Anti-AI875481 Antibody; Anti-AU022477 Antibody; anti-BID antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse p22 BID
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BID antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Mouse p22 BID Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse p22 BID (rM p22 BID). p22 BID specific IgG was purified by Mouse p22 BID affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(Mouse BID was immunoprecipitated using:Lane A:0.5 mg Raw264.7 Whole Cell Lysate1 uL anti-Mouse BID rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse BID rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

IHC (Immunohistochemisry)

(Immunochemical staining of mouse BID in mouse liver with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)

IHC (Immunohiostchemistry)

(Immunochemical staining of mouse BID in mouse kidney with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections).)

WB (Western Blot)

(Anti-BID rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-BID antibody

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Product Categories/Family for anti-BID antibody

Death Receptor Signaling

References

Gross A. (2006) BID as a double agent in cell life and death. Cell Cycle. 5(6): 582-4.Yin XM. (2007) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.Esposti MD. (2002) The roles of Bid. Apoptosis. 7(5): 433-40.Yin XM. (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10(3): 161-7.Yin XM. (2000) Bid, a critical mediator for apoptosis induced by the activation of Fas/TNF-R1 death receptors in hepatocytes. J Mol Med. 78(4): 203-11.

NCBI and Uniprot Product Information

• NCBI GI #: 148667234
• NCBI GeneID: 12122
• Molecular Weight: 21,952 Da
• NCBI Official Full Name: BH3 interacting domain death agonist, isoform CRA_a, partial
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: Bid
• NCBI Official Synonym Symbols: AI875481; AU022477; 2700049M22Rik
• NCBI Protein Information: BH3-interacting domain death agonist