IHC (Immunohistochemisry)
(Fig: 3 Two cores (A and B) from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray stained for Bid expression using at 1: 2000. Hematoxylin-eosin counterstain.)
Rabbit BID Polyclonal Antibody | anti-BID antibody
Polyclonal Antibody to BID
Gene Names
BID; FP497
Reactivity
Dog, Gerbil, Human, Mouse, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Serum
Synonyms
BID, Antibody; Polyclonal Antibody to BID; BID; p22 BID; Antibody to BID; Anti-BID Antibody; BID Antibody; anti-BID antibody
Host
Rabbit
Reactivity
Dog, Gerbil, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
195
Applicable Applications for anti-BID antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Information
A full-length recombinant protein of mouse BID was used as immunogen for this antibody
Antigen
BID
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohistochemisry)
(Fig: 3 Two cores (A and B) from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray stained for Bid expression using at 1: 2000. Hematoxylin-eosin counterstain.)
IHC (Immunohistochemisry)
(Fig: 3 Two cores (A and B) from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray stained for Bid expression using at 1: 2000. Hematoxylin-eosin counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using at 1: 2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.)
IHC (Immunohiostchemistry)
(Fig: 2 Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using at 1: 2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.)
WB (Western Blot)
(Fig: 1 Western blot analysis of Bid in normal mouse tissues using at 1: 2000. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).)
WB (Western Blot)
(Fig: 1 Western blot analysis of Bid in normal mouse tissues using at 1: 2000. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).)
Related Product Information for anti-BID antibody
The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. This antibody recognizes Bid (approx. 19-23 kDa) or cleaved/truncated forms of Bid. The C-terminal cleaved/truncated form (11-15 kDa) of Bid is often referred to as tBid in the literature (reviewed in Yin, 2006).
Product Categories/Family for anti-BID antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
11,263 Da
NCBI Official Full Name
BH3-interacting domain death agonist
NCBI Official Synonym Full Names
BH3 interacting domain death agonist
NCBI Official Symbol
BID
NCBI Official Synonym Symbols
FP497
NCBI Protein Information
BH3-interacting domain death agonist
UniProt Protein Name
BH3-interacting domain death agonist
UniProt Gene Name
BID
UniProt Synonym Gene Names
BID
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Product Notes
The BID bid (Catalog #AAA78314) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to BID reacts with Dog, Gerbil, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BID can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BID bid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BID, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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