WB (Western Blot)
(Detection of human BIG2/ARFGEF2 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 antibody AAA211272 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). BIG2/AFGEF2 was also immunoprecipitated by rabbit anti-BIG2/ARFGEF2 antibody BL4399, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
Rabbit anti-Human BIG2/ARFGEF2 Polyclonal Antibody | anti-BIG2/ARFGEF2 antibody
Rabbit anti-BIG2/ARFGEF2 Antibody, Affinity Purified
Gene Names
ARFGEF2; BIG2; PVNH2; dJ1164I10.1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BIG2/ARFGEF2, Antibody; Rabbit anti-BIG2/ARFGEF2 Antibody, Affinity Purified; ADP-ribosylation factor guanine nucleotide-exchange factor 2; PVNH2; dJ1164I10.1; brefeldin A-inhibited guanine nucleotide-exchange protein 2; brefeldin A-inhibited GEP 2; ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited); BIG2; Brefeldin A-inhibited guanine nucleotide-exchange protein 2; ADP ribosylation factor guanine nucleotide exchange factor 2; anti-BIG2/ARFGEF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-BIG2/ARFGEF2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 300 and 350
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human BIG2/ARFGEF2 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 antibody AAA211272 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). BIG2/AFGEF2 was also immunoprecipitated by rabbit anti-BIG2/ARFGEF2 antibody BL4399, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
WB (Western Blot)
(Detection of human BIG2/ARFGEF2 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 antibody AAA211272 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). BIG2/AFGEF2 was also immunoprecipitated by rabbit anti-BIG2/ARFGEF2 antibody BL4399, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human BIG2/ARFGEF2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 (Cat. No. AAA211272) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human BIG2/ARFGEF2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 (Cat. No. AAA211272) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-BIG2/ARFGEF2 antibody
BIG2/ARFGEF2 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
Product Categories/Family for anti-BIG2/ARFGEF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
202,038 Da
NCBI Official Full Name
brefeldin A-inhibited guanine nucleotide-exchange protein 2
NCBI Official Synonym Full Names
ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
NCBI Official Symbol
ARFGEF2
NCBI Official Synonym Symbols
BIG2; PVNH2; dJ1164I10.1
NCBI Protein Information
brefeldin A-inhibited guanine nucleotide-exchange protein 2; brefeldin A-inhibited GEP 2
UniProt Protein Name
Brefeldin A-inhibited guanine nucleotide-exchange protein 2
UniProt Gene Name
ARFGEF2
UniProt Synonym Gene Names
ARFGEP2; BIG2; Brefeldin A-inhibited GEP 2
UniProt Entry Name
BIG2_HUMAN
Similar Products
Product Notes
The BIG2/ARFGEF2 arfgef2 (Catalog #AAA211272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BIG2/ARFGEF2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BIG2/ARFGEF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BIG2/ARFGEF2 arfgef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BIG2/ARFGEF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.