Rabbit S-tag Polyclonal Antibody | anti-S-tag antibody

Anti-S-tag Antibody, Rabbit Polyclonal

Cat. Num. AAA254534

• Reactivity: S-tag
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: S-tag, Antibody; Anti-S-tag Antibody, Rabbit Polyclonal; Anti-S-tag Antibody, Rabbit Pab, Antigen Affinity Purified; anti-S-tag antibody

• Host: Rabbit
• Reactivity: S-tag
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Recognize N-terminal and C-terminal S tag in fusion proteins
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-S-tag antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS)
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS), and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-S Tag rabbit polyclonal antibody at 1:5000 dilutionLane A: Stag-GFERh(C) transfected 293 cell lysate at 2ugLane B: his-GFER-Stag(C) transfected 293 cell lysate at 2ugLane C: his-Stag-GFER(C) transfected 293 cell lysate at 2ugLane D: Stag-GSTT2h(C) transfected 293 cell lysate at 10ugLane E: his-Stag-GSTT2(C) transfected 293 cell lysate at 10ugLane F: his-GSTT2-Stag(C) transfected 293 cell lysate at 10ugLane G: Nagetive control at 10ugSecondaryDylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.)

IP (Immunoprecipitation)

(s-tag was immunoprecipitated using:Lane A:0.5 mg Stag-GFERh transfected 293 Whole Cell LysateLane B:0.5 mg his-GFER-Stag(C) transfected 293 Whole Cell LysateLane C:0.5 mg his-Stag-GFER(C) transfected 293 Whole Cell LysateLane D:0.5 mg Psetp2 transfected 293 Whole Cell Lysate2 uL anti-s-tag rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-s-tag rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.)

IF (Immunofluorescence)

(Immunofluorescence staining of S-Tag in Hela cells, transfected with pSTEP2-Stag-GFERh (Figure A), pSTEP2-his-GFER-Stag (Figure B), pSTEP2-his-Stag-GFER (Figure C), pSTEP2-Stag-GSTT2h (Figure D),pSTEP2-his-Stag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Stag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-S-Tag polyclonal antibody at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)

Related Product Information for anti-S-tag antibody

S-tag is a 15 amino acid truncated form of the S-peptide, KETAAAKFERQHMDS, used in a variety of protein expression applications in mammalian cells. Its small size and excessive solubility have made the S-tag an excellent fusion partner in the production of recombinant proteins. S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). The N-terminus of the original RNase A, also known as S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. On DNA-level the S-tag can be attached to the N-or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies.

References

Park JH, et al. (2001) Detection of pet-vector encoded, recombinant s-tagged proteins using the monoclonal antibody atom-2. Hybridoma 20 (1): 17-23.Murray G, et al. (2001) Calmodulin-binding protein detection using a non-radiolabeled calmodulin fusion protein. Biotechniques 30 (5): 1036-1042.Daskalow K, et al. (2008) Epitope mapping of antibodies against s-tagged fusion proteins and molecular weight markers. Biosci Biotechnol Biochem 72 (2): 346-351.