IHC (Immunohistochemisry)
(Anti- FHIT Picoband antibody, AAA46035, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Rabbit Bis(5'-adenosyl)-triphosphatase Polyclonal Antibody | anti-FHIT antibody
Anti-FHIT Antibody
Gene Names
FHIT; FRA3B; AP3Aase
Reactivity
Human. No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Bis(5'-adenosyl)-triphosphatase, Antibody; Anti-FHIT Antibody; AP3A hydrolase antibody; AP3A hydrolase fragile site 3p14.2 antibody; AP3Aase antibody; Bis 5' adenosyl triphosphatase antibody; Bis(5' adenosyl) triphosphatase antibody; Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody; Dinucleosidetriphosphatase antibody; FRA 3B antibody; FRA3B antibody; Fragile histidine triad antibody; Fragile histidine triad gene antibody; Fragile histidine triad protein antibody; Tumor suppressor protein antibody; fragile histidine triad; anti-FHIT antibody
Host
Rabbit
Reactivity
Human. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
147
Applicable Applications for anti-FHIT antibody
IHC (Immunohistochemistry), WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human FHIT recombinant protein (Position: M1-Q147). Human FHIT shares 90% and 87% amino acid (aa) sequences identity with mouse and rat FHIT, respectively.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti- FHIT Picoband antibody, AAA46035, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- FHIT Picoband antibody, AAA46035, IHC(P)IHC(P): Human Mammary Cancer Tissue)
WB (Western Blot)
(Anti- FHIT Picoband antibody, AAA46035, Western blottingAll lanes: Anti FHIT (AAA46035) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: Jurkat Whole Cell Lysate at 40ugPredicted bind size: 17KDObserved bind size: 17KD)
WB (Western Blot)
(Anti- FHIT Picoband antibody, AAA46035, Western blottingAll lanes: Anti FHIT (AAA46035) at 0.5ug/mlLane 1: HT1080 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugLane 3: Jurkat Whole Cell Lysate at 40ugPredicted bind size: 17KDObserved bind size: 17KD)
WB (Western Blot)
(Anti- FHIT Picoband antibody, AAA46035, Western blottingAll lanes: Anti FHIT (AAA46035) at 0.5ug/mlWB: Recombinant Human FHIT Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
WB (Western Blot)
(Anti- FHIT Picoband antibody, AAA46035, Western blottingAll lanes: Anti FHIT (AAA46035) at 0.5ug/mlWB: Recombinant Human FHIT Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-FHIT antibody
Description: Rabbit IgG polyclonal antibody for Bis(5'-adenosyl)-triphosphatase(FHIT) detection. Tested with WB, IHC-P in Human.
Background: Bis(5'-adenosyl)-triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
Background: Bis(5'-adenosyl)-triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,858 Da
NCBI Official Full Name
bis(5'-adenosyl)-triphosphatase
NCBI Official Synonym Full Names
fragile histidine triad
NCBI Official Symbol
FHIT
NCBI Official Synonym Symbols
FRA3B; AP3Aase
NCBI Protein Information
bis(5'-adenosyl)-triphosphatase; bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; tumor suppressor protein; dinucleosidetriphosphatase; diadenosine 5',5'''-P1,P3-triphosphate hydrolase
UniProt Protein Name
Bis(5'-adenosyl)-triphosphatase
UniProt Gene Name
FHIT
UniProt Synonym Gene Names
AP3Aase
UniProt Entry Name
FHIT_HUMAN
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Product Notes
The FHIT fhit (Catalog #AAA46035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FHIT Antibody reacts with Human. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Bis(5'-adenosyl)-triphosphatase can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the FHIT fhit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bis(5'-adenosyl)-triphosphatase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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