WB (Western Blot)
(BORIS / CTCFL antibody (pAb) tested by Western blot. 20 ug of LNCaP nuclear extract was probed with BORIS / CTCFL antibody at 1:1,000.)
Rabbit anti-Human Boris/CTCFL Polyclonal Antibody | anti-CTCFL antibody
Boris/CTCFL antibody (pAb)
Gene Names
CTCFL; CT27; BORIS; CTCF-T; HMGB1L1; dJ579F20.2
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
Boris/CTCFL, Antibody; Boris/CTCFL antibody (pAb); CT27; BORIS; CTCF-T; HMGB1L1; anti-CTCFL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-CTCFL antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This CTBP antibody was raised against a peptide within the N-terminal region of human BORIS.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(BORIS / CTCFL antibody (pAb) tested by Western blot. 20 ug of LNCaP nuclear extract was probed with BORIS / CTCFL antibody at 1:1,000.)
WB (Western Blot)
(BORIS / CTCFL antibody (pAb) tested by Western blot. 20 ug of LNCaP nuclear extract was probed with BORIS / CTCFL antibody at 1:1,000.)
ChIP (Chromatin Immunoprecipitation)
(Boris / CTCFL antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human testis and 2 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Boris / CTCFL binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
ChIP (Chromatin Immunoprecipitation)
(Boris / CTCFL antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human testis and 2 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Boris / CTCFL binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Related Product Information for anti-CTCFL antibody
Short Description: Our Boris/CTCFL antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human samples.
Background: BORIS (Brother of Regulator of Imprinted Sites)/CTCFL (CTCF-like) is a zinc-finger DNA binding protein that acts as a chromatin insulator and a paralog of the CTCF protein. Normally, BORIS/CTCFL is expressed only in primary spermatocytes and is thought to play a role in resetting DNA methylation marks during the differentiation of male germ cells. BORIS/CTCFL is also expressed in many cancers and may serve a pro-proliferative function.
Background: BORIS (Brother of Regulator of Imprinted Sites)/CTCFL (CTCF-like) is a zinc-finger DNA binding protein that acts as a chromatin insulator and a paralog of the CTCF protein. Normally, BORIS/CTCFL is expressed only in primary spermatocytes and is thought to play a role in resetting DNA methylation marks during the differentiation of male germ cells. BORIS/CTCFL is also expressed in many cancers and may serve a pro-proliferative function.
Product Categories/Family for anti-CTCFL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
76kDa
NCBI Official Full Name
transcriptional repressor CTCFL isoform 1
NCBI Official Synonym Full Names
CCCTC-binding factor like
NCBI Official Symbol
CTCFL
NCBI Official Synonym Symbols
CT27; BORIS; CTCF-T; HMGB1L1; dJ579F20.2
NCBI Protein Information
transcriptional repressor CTCFL
UniProt Protein Name
Transcriptional repressor CTCFL
UniProt Gene Name
CTCFL
UniProt Synonym Gene Names
BORIS; CT27
UniProt Entry Name
CTCFL_HUMAN
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Product Notes
The CTCFL ctcfl (Catalog #AAA59939) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Boris/CTCFL antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Boris/CTCFL can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the CTCFL ctcfl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Boris/CTCFL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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