SDS-PAGE
Transcriptional repressor CTCFL Recombinant Protein | Ctcfl recombinant protein
Recombinant Mouse Transcriptional repressor CTCFL
Gene Names
Ctcfl; Boris; OTTMUSG00000016680
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Transcriptional repressor CTCFL; N/A; Recombinant Mouse Transcriptional repressor CTCFL; Brother of the regulator of imprinted sites; CCCTC-binding factor; CTCF paralog; CTCF-like protein; Ctcfl recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-636. Full Length
Sequence
MAAAEVPVPSGYFTQIKEQKLKPGDLEEEKEEDGVQRVEAQEGVVKEVEAENSCLLLEARAPVESDRRILTLQTVHLESQDVHLQGLGWLSVPHSEELSGTVPEAEGILQLPSVLWLDPEPQLSLQHCVTVSIPEELYPPEELQRIHFHLLRENVLMAEENPELTPDLDESTALKKPEEDEKDQLPPQGETDKREERLLLLEMKPKEGKDDEIVLTISHLSLEEQQDPPAANQTSVPGAKAAKPKRRRQTKGKPQSFQCDTCPFTSSKLSTFNRHIKIHSNERPHLCHLCLKAFRTVTLLRNHVNTHTGTRPHKCRDCDMAFVTSGELVRHRRYKHTYEKPFKCSLCKYASVEASKMKRHIRSHTGERPFQCCQCAYASRDSYKLKRHMRTHSGEKPYECPTCHVRFTQSGTMKIHIAQKHGENVPKYECPHCATIIARKSDLRVHLRNLHSQSPEEMKCRYCPAGFHERYALIQHQRTHKNEKKFKCKQCDYACKQERCLKAHMRMHTGEKPFSCLACNKHFRQKQLLTVHLRKYHDPNFVPNLHLCLKCDKRFSRWSNLQRHRKKCDPEHETLAPNKDRRPVTRTQASEGEAGHKEGEPQCPGEQALGHQGEAAGSQSPDHGLTCEIIFNMMDK
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for Ctcfl recombinant protein
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Ses to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
References
BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma.Loukinov D.I., Pugacheva E., Vatolin S., Pack S.D., Moon H., Chernukhin I., Mannan P., Larsson E., Kanduri C., Vostrov A.A., Cui H., Niemitz E.L., Rasko J.E.J., Docquier F.M., Kistler M., Breen J.J., Zhuang Z., Quitschke W.W., Renkawitz R., Klenova E.M., Feinberg A.P., Ohlsson R., Morse H.C. III, Lobanenkov V.V.Proc. Natl. Acad. Sci. U.S.A. 99:6806-6811(2002) Lineage-specific biology revealed by a finished genome assembly of the mouse.Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) The testis-specific factor CTCFL cooperates with the protein methyltransferase PRMT7 in H19 imprinting control region methylation.Jelinic P., Stehle J.-C., Shaw P.PLoS Biol. 4:E355-E355(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75.1 kDa
NCBI Official Full Name
transcriptional repressor CTCFL
NCBI Official Synonym Full Names
CCCTC-binding factor (zinc finger protein)-like
NCBI Official Symbol
Ctcfl
NCBI Official Synonym Symbols
Boris; OTTMUSG00000016680
NCBI Protein Information
transcriptional repressor CTCFL
UniProt Protein Name
Transcriptional repressor CTCFL
UniProt Gene Name
Ctcfl
UniProt Synonym Gene Names
Boris
UniProt Entry Name
CTCFL_MOUSE
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Product Notes
The Ctcfl ctcfl (Catalog #AAA114087) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-636. Full Length. The amino acid sequence is listed below: MAAAEVPVPS GYFTQIKEQK LKPGDLEEEK EEDGVQRVEA QEGVVKEVEA ENSCLLLEAR APVESDRRIL TLQTVHLESQ DVHLQGLGWL SVPHSEELSG TVPEAEGILQ LPSVLWLDPE PQLSLQHCVT VSIPEELYPP EELQRIHFHL LRENVLMAEE NPELTPDLDE STALKKPEED EKDQLPPQGE TDKREERLLL LEMKPKEGKD DEIVLTISHL SLEEQQDPPA ANQTSVPGAK AAKPKRRRQT KGKPQSFQCD TCPFTSSKLS TFNRHIKIHS NERPHLCHLC LKAFRTVTLL RNHVNTHTGT RPHKCRDCDM AFVTSGELVR HRRYKHTYEK PFKCSLCKYA SVEASKMKRH IRSHTGERPF QCCQCAYASR DSYKLKRHMR THSGEKPYEC PTCHVRFTQS GTMKIHIAQK HGENVPKYEC PHCATIIARK SDLRVHLRNL HSQSPEEMKC RYCPAGFHER YALIQHQRTH KNEKKFKCKQ CDYACKQERC LKAHMRMHTG EKPFSCLACN KHFRQKQLLT VHLRKYHDPN FVPNLHLCLK CDKRFSRWSN LQRHRKKCDP EHETLAPNKD RRPVTRTQAS EGEAGHKEGE PQCPGEQALG HQGEAAGSQS PDHGLTCEII FNMMDK. It is sometimes possible for the material contained within the vial of "Transcriptional repressor CTCFL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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