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WB (Western Blot)
(Anti-BPHL rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human BPHL Polyclonal Antibody | anti-BPHL antibody
Anti-BPHL Antibody, Rabbit Polyclonal
Gene Names
BPHL; MCNAA; BPH-RP; VACVASE
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
BPHL, Antibody; Anti-BPHL Antibody, Rabbit Polyclonal; BPHL Antibody, Rabbit PAb, Antigen Affinity Purified; biphenyl hydrolase-like (serine hydrolase); Anti-BPH-RP Antibody; Anti-MCNAA Antibody; Anti-VACVASE Antibody; anti-BPHL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human BPHL
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-BPHL antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human BPHL protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human BPHL (rh BPHL). BPHL specific IgG was purified by Human BPHL affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-BPHL rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-BPHL rabbit polyclonal antibody at 1:500 dilutionLane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(BPHL was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-BPHL rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BPHL rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)
IP (Immunoprecipitation)
(BPHL was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate0.5 uL anti-BPHL rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BPHL rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 31 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human BPHL in human liver with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human BPHL in human liver with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human BPHL in human kidney with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human BPHL in human kidney with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-BPHL antibody
BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.
References
Lai L. et al., 2008, J Biol Chem. 283 (14): 9318-27. Davila S. et al., 2010, Genes Immun. 11 (3): 232-8. Hendrickson SL. et al., 2010, PLoS One. 5 (9): e12862.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,107 Da
NCBI Official Full Name
valacyclovir hydrolase isoform 1
NCBI Official Synonym Full Names
biphenyl hydrolase-like (serine hydrolase)
NCBI Official Symbol
BPHL
NCBI Official Synonym Symbols
MCNAA; BPH-RP; VACVASE
NCBI Protein Information
valacyclovir hydrolase; biphenyl hydrolase-related protein; breast epithelial mucin-associated antigen; valacyclovirase
UniProt Protein Name
Valacyclovir hydrolase
UniProt Gene Name
BPHL
UniProt Synonym Gene Names
MCNAA; VACVase; Valacyclovirase; Bph-rp; MCNAA
UniProt Entry Name
BPHL_HUMAN
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Product Notes
The BPHL bphl (Catalog #AAA255691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BPHL Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BPHL can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BPHL bphl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BPHL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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