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product-image-AAA292149_WB10.jpg WB (Western Blot) (Sample:Testis (Mouse) Lysate at 40 ugPrimary: Anti- BRD1 (bs-8426R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 119 kDObserved band size: 140 kD)

Rabbit BRD1 Polyclonal Antibody | anti-BRD1 antibody

BRD1 Polyclonal Antibody

Gene Names
BRD1; BRL; BRPF1; BRPF2
Reactivity
Rat, Dog, Cow
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BRD1, Antibody; BRD1 Polyclonal Antibody; Bromodomain-containing protein 1; BR140-like protein; Bromodomain and PHD finger-containing protein 2; BRD1; anti-BRD1 antibody
Ordering
Host
Rabbit
Reactivity
Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-BRD1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Notes
ECO:0000303|PubMed:16387653, ECO:0000312|HGNC: HGNC:1102
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Sample:Testis (Mouse) Lysate at 40 ugPrimary: Anti- BRD1 (bs-8426R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 119 kDObserved band size: 140 kD)

product-image-AAA292149_WB10.jpg WB (Western Blot) (Sample:Testis (Mouse) Lysate at 40 ugPrimary: Anti- BRD1 (bs-8426R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 119 kDObserved band size: 140 kD)

IHC (Immunohistochemisry)

(Dilution: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-BRD1 Polyclonal Antibody, Unconjugated(bs-8426R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

product-image-AAA292149_IHC11.jpg IHC (Immunohistochemisry) (Dilution: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-BRD1 Polyclonal Antibody, Unconjugated(bs-8426R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

IHC (Immunohiostchemistry)

(Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (BRD1) Polyclonal Antibody, Unconjugated (bs-8426R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

product-image-AAA292149_IHC13.jpg IHC (Immunohiostchemistry) (Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (BRD1) Polyclonal Antibody, Unconjugated (bs-8426R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

IHC (Immunohistochemistry)

(Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-BRD1 Polyclonal Antibody, Unconjugated(bs-8426R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

product-image-AAA292149_IHC15.jpg IHC (Immunohistochemistry) (Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-BRD1 Polyclonal Antibody, Unconjugated(bs-8426R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133,243 Da
NCBI Official Full Name
bromodomain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
bromodomain containing 1
NCBI Official Symbol
BRD1
NCBI Official Synonym Symbols
BRL; BRPF1; BRPF2
NCBI Protein Information
bromodomain-containing protein 1
UniProt Protein Name
Bromodomain-containing protein 1
UniProt Gene Name
BRD1
UniProt Synonym Gene Names
BRL; BRPF2
UniProt Entry Name
BRD1_HUMAN

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Product Notes

The BRD1 brd1 (Catalog #AAA292149) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRD1 Polyclonal Antibody reacts with Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's BRD1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BRD1 brd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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