Rabbit BTK Polyclonal Antibody | anti-BTK antibody

BTK Polyclonal Antibody

Cat. Num. AAA37198

• Gene Names: BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: BTK, Antibody; BTK Polyclonal Antibody; AGMX1; AT; ATK; BPK; IMD1; MGC126261; MGC126262; PSCTK1; XLA; anti-BTK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 659

• Applicable Applications for anti-BTK antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human BTK
• Calculated Molecular Weight: 76kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using BTK antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded mouse brain using BTK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded rat spleen using BTK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse spleen using BTK antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-BTK antibody

Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCbeta through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity (8).

Product Categories/Family for anti-BTK antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 547759
• NCBI GeneID: 695
• UniProt Accession #: Q06187; Q32ML5; B2RAW1
• Molecular Weight: 659
• NCBI Official Full Name: Tyrosine-protein kinase BTK
• NCBI Official Synonym Full Names: Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: BTK
• NCBI Official Synonym Symbols: AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• NCBI Protein Information: tyrosine-protein kinase BTK; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; truncated Bruton agammaglobulinemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase BTK
• UniProt Gene Name: BTK
• UniProt Synonym Gene Names: AGMX1; ATK; BPK; ATK; BPK
• UniProt Entry Name: BTK_HUMAN