WB (Western Blot)
(Western blot analysis of lysates from Raji, Ramos, K562, Daudi cell line and mouse spleen tissue lysate(from left to right), using BTK Antibody (Center). AAA286481 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Rabbit BTK Polyclonal Antibody | anti-BTK antibody
BTK Antibody (Center)
Gene Names
BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
Reactivity
Human, mouse (Predicted Reactivity: Chicken)
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BTK, Antibody; BTK Antibody (Center); Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; BTK; AGMX1; anti-BTK antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Chicken)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BTK antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 396-430 amino acids from the Central region of human BTK.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
659
Applicable Applications for anti-BTK antibody
ELISA, WB (Western Blot)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from Raji, Ramos, K562, Daudi cell line and mouse spleen tissue lysate(from left to right), using BTK Antibody (Center). AAA286481 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Raji, Ramos, K562, Daudi cell line and mouse spleen tissue lysate(from left to right), using BTK Antibody (Center). AAA286481 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Raji, Ramos, K562 cell line and mouse spleen tissue lysate(from left to right), using BTK Antibody (Center). AAA286481 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Raji, Ramos, K562 cell line and mouse spleen tissue lysate(from left to right), using BTK Antibody (Center). AAA286481 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-BTK antibody
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.
Product Categories/Family for anti-BTK antibody
References
Vetrie D.,et al.Nature 361:226-233(1993).
Vetrie D.,et al.Nature 364:362-362(1993).
Ohta Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:9062-9066(1994).
Rohrer J.,et al.Immunogenetics 40:319-324(1994).
Hagemann T.L.,et al.Hum. Mol. Genet. 3:1743-1749(1994).
Vetrie D.,et al.Nature 364:362-362(1993).
Ohta Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:9062-9066(1994).
Rohrer J.,et al.Immunogenetics 40:319-324(1994).
Hagemann T.L.,et al.Hum. Mol. Genet. 3:1743-1749(1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76281
NCBI Official Full Name
tyrosine-protein kinase BTK isoform 1
NCBI Official Synonym Full Names
Bruton agammaglobulinemia tyrosine kinase
NCBI Official Symbol
BTK
NCBI Official Synonym Symbols
AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
NCBI Protein Information
tyrosine-protein kinase BTK
UniProt Protein Name
Tyrosine-protein kinase BTK
UniProt Gene Name
BTK
UniProt Synonym Gene Names
AGMX1; ATK; BPK; ATK; BPK
UniProt Entry Name
BTK_HUMAN
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Product Notes
The BTK btk (Catalog #AAA286481) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BTK Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's BTK can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BTK btk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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