Rabbit anti-Human, Mouse Bub1 Polyclonal Antibody | anti-BUB1 antibody

Bub1 Antibody

Cat. Num. AAA40026

• Gene Names: BUB1; BUB1A; BUB1L; hBUB1
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: Bub1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Bub1, Antibody; Bub1 Antibody; Bub1; BUB1A; BUB1L; hBUB1; budding uninhibited by benzimidazoles 1 homolog (yeast); anti-BUB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Bub1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1085

• Applicable Applications for anti-BUB1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.
• Buffer: Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Bub1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 μg/mL.)

WB (Western Blot)

(Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 μg/mL.)

Related Product Information for anti-BUB1 antibody

Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 8134347
• NCBI GeneID: 699
• UniProt Accession #: O43683; O43430; O43643; O60626; Q53QE4; E9PC26; F5GXI5
• Molecular Weight: 120,002 Da
• NCBI Official Full Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• NCBI Official Synonym Full Names: BUB1 mitotic checkpoint serine/threonine kinase
• NCBI Official Symbol: BUB1
• NCBI Official Synonym Symbols: BUB1A; BUB1L; hBUB1
• NCBI Protein Information: mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog
• UniProt Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• UniProt Gene Name: BUB1
• UniProt Synonym Gene Names: BUB1L; hBUB1
• UniProt Entry Name: BUB1_HUMAN