Application Data
(Anti-Bub1 Picoband antibody, AAA46033-2.jpgAll lanes: Anti BUB1 (AAA46033) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Ovary Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 122KDObserved bind size: 122KD)
Rabbit Mitotic checkpoint serine/threonine-protein kinase BUB1 Polyclonal Antibody | anti-BUB1 antibody
Anti-Bub1 Antibody
Gene Names
BUB1; BUB1A; BUB1L; hBUB1
Reactivity
Human, Rat. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Mitotic checkpoint serine/threonine-protein kinase BUB1, Antibody; Anti-Bub1 Antibody; Bub1 antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody; BUB1 mitotic checkpoint serine/threonine kinase antibody; BUB1, S. cerevisiae, homolog of antibody; BUB1_HUMAN antibody; BUB1A antibody; BUB1L antibody; Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody; Budding uninhibited by benzimidazoles 1 homolog antibody; Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody; hBUB1 antibody; Homolog of mitotic checkpoint gene BUB1 antibody; Mitotic checkpoint gene BUB1 antibody; Mitotic checkpoint serine/threonine protein kinase BUB1 antibody; Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody; Mitotic spindle checkpoint kinase antibody; Putative serine/threonine protein kinase antibody; BUB1 mitotic checkpoint serine/threonine kinase; anti-BUB1 antibody
Host
Rabbit
Reactivity
Human, Rat. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
1028
Applicable Applications for anti-BUB1 antibody
WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Application Data
(Anti-Bub1 Picoband antibody, AAA46033-2.jpgAll lanes: Anti BUB1 (AAA46033) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Ovary Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 122KDObserved bind size: 122KD)
Application Data
(Anti-Bub1 Picoband antibody, AAA46033-2.jpgAll lanes: Anti BUB1 (AAA46033) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Ovary Tissue Lysate at 50ugLane 3: Rat Liver Tissue Lysate at 50ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugLane 6: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 122KDObserved bind size: 122KD)
Application Data
(Anti-Bub1 Picoband antibody, AAA46033-1.jpgAll lanes: Anti BUB1 (AAA46033) at 0.5ug/mlWB: Recombinant Human BUB1 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Application Data
(Anti-Bub1 Picoband antibody, AAA46033-1.jpgAll lanes: Anti BUB1 (AAA46033) at 0.5ug/mlWB: Recombinant Human BUB1 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-BUB1 antibody
Description: Rabbit IgG polyclonal antibody for Mitotic checkpoint serine/threonine-protein kinase BUB1(BUB1) detection. Tested with WB in Human;Rat.
Background: BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.
Background: BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120,002 Da
NCBI Official Full Name
mitotic checkpoint serine/threonine-protein kinase BUB1 isoform 3
NCBI Official Synonym Full Names
BUB1 mitotic checkpoint serine/threonine kinase
NCBI Official Symbol
BUB1
NCBI Official Synonym Symbols
BUB1A; BUB1L; hBUB1
NCBI Protein Information
mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog
UniProt Protein Name
Mitotic checkpoint serine/threonine-protein kinase BUB1
UniProt Gene Name
BUB1
UniProt Synonym Gene Names
BUB1L; hBUB1
UniProt Entry Name
BUB1_HUMAN
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Product Notes
The BUB1 bub1 (Catalog #AAA46033) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bub1 Antibody reacts with Human, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Mitotic checkpoint serine/threonine-protein kinase BUB1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the BUB1 bub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitotic checkpoint serine/threonine-protein kinase BUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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