Rabbit c-Cbl Polyclonal Antibody | anti-CBL antibody

Phospho-c-Cbl (Tyr700) Antibody

Cat. Num. AAA321312

• Gene Names: CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Synonyms: c-Cbl, Antibody; Phospho-c-Cbl (Tyr700) Antibody; 4732447J05Rik; C CBL; Cas Br M (murine) ecotropic retroviral transforming sequence; Casitas B lineage lymphoma proto oncogene; Casitas B-lineage lymphoma proto-oncogene; CBL 2; cbl; CBL_HUMAN; CBL2; E3 ubiquitin protein ligase CBL; E3 ubiquitin-protein ligase CBL; Oncogene CBL2; Proto oncogene c CBL; Proto-oncogene c-CBL; RGD1561386; RING finger protein 55; RNF55; Signal transduction protein CBL; anti-CBL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-c-Cbl (Tyr700) antibody detects endogenous levels of c-Cbl.
• Purity/Purification: From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 906

• Applicable Applications for anti-CBL antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human c-Cbl around the phosphorylation site of Tyr700.
• Subcellular Location: Cytoplasm.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Similarity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%), Chicken (82%)
• Conjugation: Unconjugated
• Epitope: Phospho Tyr700
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts of Jurkat treated with pervanadate, using Phospho-c-Cbl (Tyr700) Antibody.)

IHC (Immunohistochemistry)

(AAA321312 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-CBL antibody

Function: Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
Subunit Structure: Interacts (phosphorylated at Tyr-731) with PIK3R1. Associates with NCK via its SH3 domain. The phosphorylated C-terminus interacts with CD2AP via its second SH3 domain. Binds to UBE2L3. Interacts with adapters SLA, SLA2 and with the phosphorylated C-terminus of SH2B2. Interacts with EGFR, SYK and ZAP70 via the highly conserved Cbl-N region. Also interacts with SORBS1 and INPPL1/SHIP2. Interacts with phosphorylated LAT2. May interact with CBLB (By similarity). Interacts with ALK, AXL, BLK, FGR and FGFR2. Interacts with CSF1R, EPHB1, FLT1, KDR, PDGFRA and PDGFRB; regulates receptor degradation through ubiquitination. Interacts with HCK and LYN. Interacts with TEK/TIE2 (tyrosine phosphorylated). Interacts with SH3KBP1 and this interaction is inhibited in the presence of SHKBP1 (By similarity). Interacts with SIGLEC10 (By similarity).
Post-translational Modifications: Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK. Ubiquitinated, leading to its degradation via the proteasome.
Similarity: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

NCBI and Uniprot Product Information

• NCBI GI #: 52426745
• NCBI GeneID: 867
• NCBI Accession #: NP_005179.2
• NCBI GenBank Nucleotide #: NM_005188.3
• UniProt Accession #: P22681; A3KMP8
• Molecular Weight: Observed: 120 kDa; Predicted: 100 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL
• NCBI Official Synonym Full Names: Cbl proto-oncogene
• NCBI Official Symbol: CBL
• NCBI Official Synonym Symbols: CBL2; NSLL; C-CBL; RNF55; FRA11B
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL
• UniProt Gene Name: CBL
• UniProt Synonym Gene Names: CBL2; RNF55