WB (Western Blot)
(Detection of human C/EBP beta by western blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), HEK293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody AAA211994 (lot AAA211994-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human C/EBP beta Polyclonal Antibody | anti-C/EBPbeta antibody
Rabbit anti-C/EBP beta Antibody, Affinity Purified
Gene Names
CEBPB; LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
C/EBP beta, Antibody; Rabbit anti-C/EBP beta Antibody, Affinity Purified; CCAAT/enhancer binding protein (C/EBP), beta; Liver-enriched inhibitory protein; transcription factor 5; TCF-5; TCF5; transcription factor C/EBP beta; nuclear factor of interleukin 6; nuclear factor NF-IL6; NF-IL6; Liver activator protein; LIP; LAP; interleukin 6-dependent DNA-binding protein; CCAAT/enhancer-binding protein beta; C/EBP-beta; C/EBP beta; IL6DBP; CCAAT enhancer binding protein beta; anti-C/EBPbeta antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-C/EBPbeta antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Bovine
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human C/EBP beta by western blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), HEK293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody AAA211994 (lot AAA211994-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human C/EBP beta by western blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), HEK293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody AAA211994 (lot AAA211994-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human C/EBP beta by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody AAA211994 (lot AAA211994-2) used for IP at 6 ug per reaction. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody For blotting immunoprecipitated C/EBP beta, AAA211994 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.linelineline)
IP (Immunoprecipitation)
(Detection of human C/EBP beta by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody AAA211994 (lot AAA211994-2) used for IP at 6 ug per reaction. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody For blotting immunoprecipitated C/EBP beta, AAA211994 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.linelineline)
IHC (Immunohistochemistry)
(Detection of human C/EBP beta by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta (Cat. No. AAA211994 Lot2) used at a dilution of 1:1,000 (1ug/ml) Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human C/EBP beta by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta (Cat. No. AAA211994 Lot2) used at a dilution of 1:1,000 (1ug/ml) Detection: DAB)
Related Product Information for anti-C/EBPbeta antibody
CCAAT/enhancer binding protein beta (C/EBP beta) is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. C/EBP beta is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes [taken from NCBI Entrez Gene (GeneID: 1051)].
Product Categories/Family for anti-C/EBPbeta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,106 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein beta
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), beta
NCBI Official Symbol
CEBPB
NCBI Official Synonym Symbols
LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta
NCBI Protein Information
CCAAT/enhancer-binding protein beta; TCF-5; nuclear factor NF-IL6; transcription factor 5; nuclear factor of interleukin 6; interleukin 6-dependent DNA-binding protein; liver-enriched transcriptional activator protein
UniProt Protein Name
CCAAT/enhancer-binding protein beta
UniProt Gene Name
CEBPB
UniProt Synonym Gene Names
LAP; TCF5; C/EBP beta; TCF-5
UniProt Entry Name
CEBPB_HUMAN
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Product Notes
The C/EBPbeta cebpb (Catalog #AAA211994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-C/EBP beta Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP beta can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the C/EBPbeta cebpb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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