FCM/FACS (Flow Cytometry)
(C12orf48 Antibody (C-term)(AAA290342) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human C12orf48 Polyclonal Antibody | anti-PARPBP antibody
C12orf48 Antibody (C-term)
Gene Names
PARPBP; AROM; PARI; C12orf48
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
C12orf48, Antibody; C12orf48 Antibody (C-term); PCNA-interacting partner; PARI; PARP-1 binding protein; PARP1-binding protein; PARPBP; C12orf48; anti-PARPBP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This C12orf48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 522-550 amino acids from the C-terminal region of human C12orf48.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
522-550
Sequence Length
302
Applicable Applications for anti-PARPBP antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Binds single-strand DNA and poly(A) homopolymers. Positively regulate the poly(ADP-ribosyl)ation activity of PARP1; however such function may be indirect.
Cellular Location
Cytoplasm. Nucleus Note=Localizes to chromatin in response to S phase arrest but not in mitosis. Targeted to chromatin via its interaction with PCNA
Tissue Location
Restricted to testis. Overexpressed in multiple cancer cells.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(C12orf48 Antibody (C-term)(AAA290342) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(C12orf48 Antibody (C-term)(AAA290342) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(C12orf48 antibody (C-term)(AAA290342) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C12orf48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(C12orf48 antibody (C-term)(AAA290342) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C12orf48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(C12orf48 Antibody (C-term)(AAA290342) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C12orf48 antibody detected the FBXO34 protein (arrow).)
WB (Western Blot)
(C12orf48 Antibody (C-term)(AAA290342) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C12orf48 antibody detected the FBXO34 protein (arrow).)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65054
NCBI Official Full Name
PCNA-interacting partner
NCBI Official Synonym Full Names
PARP1 binding protein
NCBI Official Symbol
PARPBP
NCBI Official Synonym Symbols
AROM; PARI; C12orf48
NCBI Protein Information
PCNA-interacting partner
UniProt Protein Name
PCNA-interacting partner
UniProt Gene Name
PARPBP
UniProt Synonym Gene Names
C12orf48; PARI; PARI; PARPBP
UniProt Entry Name
PARI_HUMAN
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Product Notes
The PARPBP parpbp (Catalog #AAA290342) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 522-550. The C12orf48 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C12orf48 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PARPBP parpbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C12orf48, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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