anti-Human PARPBP / C12orf48 Polyclonal Antibody | anti-PARPBP / C12orf48 antibody

Anti-PARPBP / C12orf48 Antibody (aa522-550) IHC-plus

Cat. Num. AAA52612

• Gene Names: PARPBP; AROM; PARI; C12orf48
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Purity: Protein A purified
• Synonyms: PARPBP / C12orf48, Antibody; Anti-PARPBP / C12orf48 Antibody (aa522-550) IHC-plus; Rabbit Polyclonal to Human PARPBP / C12orf48; Human PARPBP / C12orf48; AROM; PARP1-binding protein; PARI; PARP-1 binding protein; PARP1 binding protein; PCNA-interacting partner; anti-PARPBP / C12orf48 antibody

• Host: PARPBP/C12orf48 antibody was produced in Rabbit.
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This C12orf48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 522-550 amino acids from the C-terminal region of human C12orf48.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 579

• Applicable Applications for anti-PARPBP / C12orf48 antibody: FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen: PARPBP / C12orf48 antibody was raised against Human.
• Epitope: aa522-550
• Note: During shipment, small volumes of product will occasionally become entraped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months.; For long term storage store at -20°C.

FCM/FACS (Flow Cytometry)

(C12orf48 Antibody flow cytometry of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(C12orf48 Antibody western blot of A549 cell line lysates (35 ug/lane). The C12orf48 antibody detected the FBXO34 protein (arrow).)

IHC (Immunohistochemistry)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-PARPBP / C12orf48 antibody

PARPBP Antibody, AROM Antibody, C12orf48 Antibody, PARP1-binding protein Antibody, PARI Antibody, PARP-1 binding protein Antibody, PARP1 binding protein Antibody, PCNA-interacting partner Antibody Description: Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Binds single-strand DNA and poly(A) homopolymers. Positively regulate the poly(ADP-ribosyl)ation activity of PARP1; however such function may be indirect.

NCBI and Uniprot Product Information

• NCBI GI #: 300797388
• NCBI GeneID: 55010
• NCBI Accession #: NP_060385.3
• NCBI GenBank Nucleotide #: NM_017915.4
• UniProt Accession #: Q9NWS1; Q05C00; Q499L8; Q4FZA0; Q4KMW7; Q6NSC6; Q6PJA1; Q86W36; B4E0Y0
• NCBI Official Full Name: PCNA-interacting partner isoform 2
• NCBI Official Synonym Full Names: PARP1 binding protein
• NCBI Official Symbol: PARPBP
• NCBI Official Synonym Symbols: AROM; PARI; C12orf48
• NCBI Protein Information: PCNA-interacting partner
• UniProt Protein Name: PCNA-interacting partner
• UniProt Gene Name: PARPBP
• UniProt Synonym Gene Names: C12orf48; PARI; PARI; PARPBP
• UniProt Entry Name: PARI_HUMAN