WB (Western Blot)
(Detection of human cAbl by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-cAbl antibody AAA211576 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. cAbl was also immunoprecipitated by rabbit anti-cAbl antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
Rabbit anti-Human cAbl Polyclonal Antibody | anti-cAbl antibody
Rabbit anti-cAbl Antibody, Affinity Purified
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; bcr/abl
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
cAbl, Antibody; Rabbit anti-cAbl Antibody, Affinity Purified; p150; proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; tyrosine-protein kinase ABL1; v-abl; v-abl Abelson murine leukemia viral oncogene homolog 1; JTK7; c-ABL; Abelson murine leukemia viral oncogene homolog 1; c-abl oncogene 1, receptor tyrosine kinase; c-ABL1; bcr/c-abl oncogene protein; bcr/abl; ABL; Abelson tyrosine-protein kinase 1; CHDSKM; Tyrosine-protein kinase ABL1; ABL proto-oncogene 1, non-receptor tyrosine kinase; anti-cAbl antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-cAbl antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 550 and 600
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human cAbl by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-cAbl antibody AAA211576 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. cAbl was also immunoprecipitated by rabbit anti-cAbl antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human cAbl by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-cAbl antibody AAA211576 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. cAbl was also immunoprecipitated by rabbit anti-cAbl antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human cAbl by immunohistochemistry and immunocytochemistry. Sample: FFPE section of human prostate carcinoma (left) and formaldehyde-fixed K-562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene) (right). Antibody: Affinity purified rabbit anti-cAbl (Cat. No.AAA211576) used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human cAbl by immunohistochemistry and immunocytochemistry. Sample: FFPE section of human prostate carcinoma (left) and formaldehyde-fixed K-562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene) (right). Antibody: Affinity purified rabbit anti-cAbl (Cat. No.AAA211576) used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-cAbl antibody
c-Abl is the human cellular homolog of a the v-Abl viral oncogene. v-Abl was originally discovered as a mouse cell-derived sequence in the genome of the Abelson murine leukemia virus (A-MuLV), a transforming retrovirus isolated by the laboratory of Dr. H.T. Abelson. c-Abl is a tyrosine kinase from the Src-family of tyrosine kinases that localizes to both the cytoplasm and nucleus. It bears an SH2 (src-homology 2) and SH3 (src-homology 3) domain responsible for mediating c-Abl protein-protein interactions. c-Abl is implicated to play a role in multiple cellular processes such as cell cycle checkpoint signaling, apoptosis, cell differentiation, cell adhesion, and transcriptional regulation. Chromosomal translocations involving the c-Abl gene are associated with human leukemias.
Product Categories/Family for anti-cAbl antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,873 Da
NCBI Official Full Name
tyrosine-protein kinase ABL1 isoform a
NCBI Official Synonym Full Names
c-abl oncogene 1, non-receptor tyrosine kinase
NCBI Official Symbol
ABL1
NCBI Official Synonym Symbols
ABL; JTK7; p150; c-ABL; v-abl; bcr/abl
NCBI Protein Information
tyrosine-protein kinase ABL1; proto-oncogene c-Abl; bcr/c-abl oncogene protein; Abelson tyrosine-protein kinase 1; c-abl oncogene 1, receptor tyrosine kinase; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolo
UniProt Protein Name
Tyrosine-protein kinase ABL1
UniProt Gene Name
ABL1
UniProt Synonym Gene Names
ABL; JTK7
UniProt Entry Name
ABL1_HUMAN
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Product Notes
The cAbl abl1 (Catalog #AAA211576) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-cAbl Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's cAbl can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the cAbl abl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cAbl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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