WB (Western Blot)
(Detection of human and mouse CacyBP by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody AAA212069 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
Rabbit anti-Human, Mouse CacyBP Polyclonal Antibody | anti-CacyBP antibody
Rabbit anti-CacyBP Antibody, Affinity Purified
Gene Names
CACYBP; SIP; GIG5; PNAS-107; S100A6BP
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CacyBP, Antibody; Rabbit anti-CacyBP Antibody, Affinity Purified; S100A6-binding protein; SIP; Siah-interacting protein (SIP); S100A6BP; PNAS-107; hCacyBP; growth-inhibiting gene 5 protein; GIG5; calcyclin-binding protein; CacyBP; Siah-interacting protein; Calcyclin-binding protein; calcyclin binding protein; anti-CacyBP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CacyBP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 178 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse CacyBP by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody AAA212069 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human and mouse CacyBP by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody AAA212069 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human CacyBP by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CacyBP (Cat. No. AAA212069 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human CacyBP by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CacyBP (Cat. No. AAA212069 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-CacyBP antibody
Calcyclin binding protein (CacyBP) is a protein that binds calcyclin. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin [taken from NCBI Entrez Gene (Gene ID: 27101].
Product Categories/Family for anti-CacyBP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,228 Da
NCBI Official Full Name
Homo sapiens calcyclin binding protein (CACYBP), transcript variant 1, mRNA
NCBI Official Synonym Full Names
calcyclin binding protein
NCBI Official Symbol
CACYBP
NCBI Official Synonym Symbols
SIP; GIG5; PNAS-107; S100A6BP
NCBI Protein Information
calcyclin-binding protein; S100A6-binding protein; Siah-interacting protein (SIP); growth-inhibiting gene 5 protein; hCacyBP
UniProt Protein Name
Calcyclin-binding protein
UniProt Gene Name
CACYBP
UniProt Synonym Gene Names
S100A6BP; SIP; CacyBP; hCacyBP
UniProt Entry Name
CYBP_HUMAN
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Product Notes
The CacyBP cacybp (Catalog #AAA212069) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CacyBP Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CacyBP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CacyBP cacybp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CacyBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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