Rabbit anti-Mouse CAD Polyclonal Antibody | anti-Dffb antibody

CAD Antibody

Cat. Num. AAA39628

• Gene Names: Dffb; CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
• Reactivity: Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: CAD Antibody is affinity chromatography purified via peptide column.
• Synonyms: CAD, Antibody; CAD Antibody; CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik; Cad; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; DNA fragmentation factor, beta subunit; anti-Dffb antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CAD Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 344

• Applicable Applications for anti-Dffb antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: CAD antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CAD.
• Buffer: CAD Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CAD antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.Green: CAD antibody (2011)Red: Phylloidin stainingBlue: DAPI staining)

IHC (Immunohistochemistry)

(Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 1 μg/mL.)

WB (Western Blot)

(Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution.)

Related Product Information for anti-Dffb antibody

CAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 20137695
• NCBI GeneID: 13368
• UniProt Accession #: O54788
• Molecular Weight: 40 kDa
• NCBI Official Full Name: DNA fragmentation factor subunit beta
• NCBI Official Synonym Full Names: DNA fragmentation factor, beta subunit
• NCBI Official Symbol: Dffb
• NCBI Official Synonym Symbols: CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
• NCBI Protein Information: DNA fragmentation factor subunit beta; DFF-40; caspase-activated DNase; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DNase inhibited by DNA fragmentation factor; DNA fragmentation factor, 40 kD, beta subunit
• UniProt Protein Name: DNA fragmentation factor subunit beta
• UniProt Gene Name: Dffb
• UniProt Synonym Gene Names: Cad; CAD; Caspase-activated DNase; DFF-40
• UniProt Entry Name: DFFB_MOUSE