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FCM/FACS (Flow Cytometry)
(DFFB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human DFFB Polyclonal Antibody | anti-DFFB antibody
DFFB Antibody (N-term)
Gene Names
DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DFFB, Antibody; DFFB Antibody (N-term); DNA fragmentation factor subunit beta; 3---; Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated nuclease; CPAN; DNA fragmentation factor 40 kDa subunit; DFF-40; DFFB; DFF2; DFF40; anti-DFFB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DFFB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
103
Applicable Applications for anti-DFFB antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(DFFB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DFFB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(DFFB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(DFFB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of DFFB Antibody (N-term) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of DFFB Antibody (N-term) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.)
WB (Western Blot)
(All lanes : Anti-DFFB Antibody (N-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: Jurkat whole cell lysatesLane 4: LNCaP whole cell lysatesLane 5: Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DFFB Antibody (N-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: Jurkat whole cell lysatesLane 4: LNCaP whole cell lysatesLane 5: Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DFFB antibody
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Product Categories/Family for anti-DFFB antibody
References
Hanus, J., et al. Apoptosis 13(3):377-382(2008)
Kalinowska-Herok, M., et al. Acta Biochim. Pol. 55(1):21-26(2008)
Neimanis, S., et al. J. Biol. Chem. 282(49):35821-35830(2007)
Hristoskova, S., et al. J. Cell. Physiol. 213(2):490-494(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Kalinowska-Herok, M., et al. Acta Biochim. Pol. 55(1):21-26(2008)
Neimanis, S., et al. J. Biol. Chem. 282(49):35821-35830(2007)
Hristoskova, S., et al. J. Cell. Physiol. 213(2):490-494(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39110
NCBI Official Full Name
DNA fragmentation factor subunit beta isoform 2
NCBI Official Synonym Full Names
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
NCBI Official Symbol
DFFB
NCBI Official Synonym Symbols
CAD; CPAN; DFF2; DFF40; DFF-40
NCBI Protein Information
DNA fragmentation factor subunit beta
UniProt Protein Name
DNA fragmentation factor subunit beta
UniProt Gene Name
DFFB
UniProt Synonym Gene Names
CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40
UniProt Entry Name
DFFB_HUMAN
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Product Notes
The DFFB dffb (Catalog #AAA289339) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The DFFB Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DFFB can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DFFB dffb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DFFB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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