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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CALM1 Antibody (C-term) (AAA290034) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit CALM1 Polyclonal Antibody | anti-CALM1 antibody
CALM1 Antibody (C-term)
Gene Names
CALM2; caM; PHKD; CAMII; LQT15; PHKD2
Reactivity
Predicted Reactivity: Human, Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Xenopus, Sheep
Applications
Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CALM1, Antibody; CALM1 Antibody (C-term); Calmodulin; CaM; CALM1; CALM; CAM; CAM1; anti-CALM1 antibody
Host
Rabbit
Reactivity
Predicted Reactivity: Human, Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Xenopus, Sheep
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CALM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-132 amino acids from the C-terminal region of human CALM1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
149
Applicable Applications for anti-CALM1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Region
107-132
Cellular location
Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Note - Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules
Note - Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules
Function
Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins throughcalcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed: ). Is a regulator of voltage-dependent L-typecalcium channels (PubMed: ). Mediates calcium-dependent inactivation of CACNA1C (PubMed: ).Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed: ). Forms a potassium channel complex with KCNQ1 and regulates electrophysiological activity of the channel via calcium-binding (PubMed: ). Acts as a sensor to modulate the endomplasmic reticulum contacts with other organelles mediated by VMP1:ATP2A2 (PubMed: ).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CALM1 Antibody (C-term) (AAA290034) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CALM1 Antibody (C-term) (AAA290034) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohistochemisry)
(CALM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) (AAA290034) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(CALM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) (AAA290034) for immunohistochemistry. Clinical relevance has not been evaluated.)
FCM/FACS (Flow Cytometry)
(CALM1 Antibody (C-term) (AAA290034) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CALM1 Antibody (C-term) (AAA290034) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CALM1 Antibody (C-term) (AAA290034) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml))
WB (Western Blot)
(Western blot analysis of CALM1 Antibody (C-term) (AAA290034) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml))
Related Product Information for anti-CALM1 antibody
CALM1 is a member of the EF-hand calcium-binding protein family.Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Product Categories/Family for anti-CALM1 antibody
References
Zhao,D., et.al., Zhonghua Yi Xue Za Zhi 88 (35), 2452-2456 (2008)
Martins-de-Souza,D., et.al., J. Neural Transm. 116 (3), 275-289 (2009)
Martins-de-Souza,D., et.al., J. Neural Transm. 116 (3), 275-289 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
calmodulin isoform 2
NCBI Official Synonym Full Names
calmodulin 2 (phosphorylase kinase, delta)
NCBI Official Symbol
CALM2
NCBI Official Synonym Symbols
caM; PHKD; CAMII; LQT15; PHKD2
NCBI Protein Information
calmodulin
UniProt Protein Name
Calmodulin
UniProt Gene Name
CALM1
UniProt Synonym Gene Names
CALM; CAM; CAM1; CaM
UniProt Entry Name
CALM_HUMAN
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Product Notes
The CALM1 calm1 (Catalog #AAA290034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CALM1 Antibody (C-term) reacts with Predicted Reactivity: Human, Bovine, C Elegans, Chicken, Zebrafish, Drosophila, Mouse, Rabbit, Rat, Xenopus, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's CALM1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CALM1 calm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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