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ICC (Immunocytochemistry)
(ICC staining Calpain 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit Calpain 1 Polyclonal Antibody | anti-CANP1 antibody
Calpain 1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Calpain 1; Polyclonal Antibody; Calpain 1 Antibody; Ca2 activated neutral protease antibody; Calcium activated neutral proteinase antibody; Calcium activated neutral proteinase small subunit antibody; Calcium dependent protease small subunit 1 antibody; Calcium dependent protease small subunit antibody; Calcium-activated neutral proteinase 1 antibody; Calpain 1 antibody; Calpain 1 large subunit antibody; (mu/I) large subunit antibody; Calpain mu type antibody; Calpain mu-type antibody; Calpain regulatory subunit antibody; Calpain small subunit 1 antibody; Calpain; large polypeptide L1 antibody; Calpain-1 catalytic subunit antibody; Calpain-1 large subunit antibody; CAN1_HUMAN antibody; CANP 1 antibody; CANP antibody; CANP small subunit antibody; CANP1 antibody; CANPL 1 antibody; CANPL1 antibody; CAPN 1 antibody; CAPN1 antibody; Cell proliferation inducing protein 30 antibody; Cell proliferation-inducing gene 30 protein antibody; Micromolar Calpain antibody; Micromolar-calpain antibody; Mu Calpain antibody; muCANP antibody; muCL antibody; anti-CANP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CANP1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
WB: 1:500
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Calpain 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Calpain 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Calpain 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Calpain 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Calpain 1 on HUVEC cell lysate using anti-Calpain 1 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of Calpain 1 on HUVEC cell lysate using anti-Calpain 1 antibody at 1/500 dilution.)
Related Product Information for anti-CANP1 antibody
Calpain 1, also designated -calpain, is an intracellular calcium-dependent protease that cleaves cyto-skeletal and submembranous proteins. Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
Product Categories/Family for anti-CANP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
81,890 Da
NCBI Official Full Name
calpain 1, (mu/I) large subunit, isoform CRA_c
NCBI Official Synonym Full Names
calpain 1, (mu/I) large subunit
NCBI Official Symbol
CAPN1
NCBI Official Synonym Symbols
CANP; muCL; CANP1; CANPL1; muCANP
NCBI Protein Information
calpain-1 catalytic subunit; CANP 1; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calpain, large polypeptide L1; calcium-activated neutral proteinase 1; cell proliferation-inducing protein 30; cell proliferation-inducing gene 30 protein
UniProt Protein Name
Calpain-1 catalytic subunit
UniProt Gene Name
CAPN1
UniProt Synonym Gene Names
CANPL1; CANP 1; muCANP
UniProt Entry Name
CAN1_HUMAN
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Product Notes
The CANP1 capn1 (Catalog #AAA29883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calpain 1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calpain 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). WB: 1:500 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CANP1 capn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calpain 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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