WB (Western Blot)
(Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.)
Rabbit CAMK2 / CAMKII Polyclonal Antibody | anti-CAMK2 antibody
Rabbit Polyclonal to Rat CAMK2 / CAMKII
Reactivity
Xenopus, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
CAMK2 / CAMKII, Antibody; Rabbit Polyclonal to Rat CAMK2 / CAMKII; Anti-CAMK2 / CAMKII Antibody (phospho-Thr286) IHC-plus; Rat CAMK2; CAMKII; anti-CAMK2 antibody
Host
Rabbit
Reactivity
Xenopus, Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment
Purity/Purification
Immunoaffinity Purified
Form/Format
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA, 50% glycerol
Applicable Applications for anti-CAMK2 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Rat
Immunogen Description
Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in rat brain CaM Kinase II.
Immunogen
CAMK2 / CAMKII antibody was raised against phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in rat brain CaM Kinase II.
Antigen Modification
pThr286
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.)
WB (Western Blot)
(Western blot of rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k b-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with l-Ptase.)
IHC (Immunohistochemistry)
(Anti-CAMK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
IHC (Immunohistochemistry)
(Anti-CAMK2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
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Product Notes
The CAMK2 (Catalog #AAA50894) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Rat CAMK2 / CAMKII reacts with Xenopus, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CAMK2 / CAMKII can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CAMK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMK2 / CAMKII, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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