WB (Western Blot)
(Sample:Lane 1: U251 (Human) Cell Lysate at 30 ugLane 2: U87MG (Human) Cell Lysate at 30 ugLane 3: Huvec (Human) Cell Lysate at 30 ugPrimary: Anti-CAPRIN2 (bs-12423R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120-140 kDObserved band size: 140 kD)
Rabbit anti-Rat, Pig CAPRIN2 Polyclonal Antibody | anti-CAPRIN2 antibody
CAPRIN2 Polyclonal Antibody
WB (Western Blot)
(Sample:Lane 1: U251 (Human) Cell Lysate at 30 ugLane 2: U87MG (Human) Cell Lysate at 30 ugLane 3: Huvec (Human) Cell Lysate at 30 ugPrimary: Anti-CAPRIN2 (bs-12423R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120-140 kDObserved band size: 140 kD)
WB (Western Blot)
(Sample:Lane 1: U251 (Human) Cell Lysate at 30 ugLane 2: U87MG (Human) Cell Lysate at 30 ugLane 3: Huvec (Human) Cell Lysate at 30 ugPrimary: Anti-CAPRIN2 (bs-12423R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120-140 kDObserved band size: 140 kD)
FCM/FACS (Flow Cytometry)
(Dilution: Blank control: Mouse spleen cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-CAPRIN2 antibody(bs-12423R), Dilution: 5 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
FCM/FACS (Flow Cytometry)
(Dilution: Blank control: Mouse spleen cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-CAPRIN2 antibody(bs-12423R), Dilution: 5 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (bs-12423R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohiostchemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated (bs-12423R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
WB (Western Blot)
(Sample:U87MG(Human) Cell Lysate at 30 ugU2OS(Human) Cell Lysate at 30 ugPrimary: Anti- CAPRIN2 (bs-12423R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 126 kDObserved band size: 126 kD)
WB (Western Blot)
(Sample:U87MG(Human) Cell Lysate at 30 ugU2OS(Human) Cell Lysate at 30 ugPrimary: Anti- CAPRIN2 (bs-12423R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 126 kDObserved band size: 126 kD)
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CAPRIN2 caprin2 (Catalog #AAA291406) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CAPRIN2 Polyclonal Antibody reacts with Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CAPRIN2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CAPRIN2 caprin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAPRIN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.