FCM/FACS (Flow Cytometry)
(CASP2 Antibody (Center) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CASP2 Polyclonal Antibody | anti-CASP2 antibody
CASP2 Antibody (Center)
Gene Names
CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CASP2, Antibody; CASP2 Antibody (Center); Caspase-2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12; CASP2; ICH1; NEDD2; anti-CASP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the Central region of human CASP2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
198-226
Sequence Length
91
Applicable Applications for anti-CASP2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CASP2 Antibody (Center) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CASP2 Antibody (Center) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung with CASP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung with CASP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of CASP2 antibody (Center) in HL60, K562, Ramos and NIH-3T3 cell line lysates(35ug/lane). CASP2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CASP2 antibody (Center) in HL60, K562, Ramos and NIH-3T3 cell line lysates(35ug/lane). CASP2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CASP2 antibody
CASP2 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. The protein exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli.
Product Categories/Family for anti-CASP2 antibody
References
Lan,Q., Morton,L.M. Blood (2009) In press
Shi,M., Vivian,C.J. Cell 136 (3), 508-520 (2009)
Paroni,G., Henderson,C. J. Biol. Chem. 276 (24), 21907-21915 (2001)
Tiso,N., Pallavicini,A. Biochem. Biophys. Res. Commun. 225 (3), 983-989 (1996)
Shi,M., Vivian,C.J. Cell 136 (3), 508-520 (2009)
Paroni,G., Henderson,C. J. Biol. Chem. 276 (24), 21907-21915 (2001)
Tiso,N., Pallavicini,A. Biochem. Biophys. Res. Commun. 225 (3), 983-989 (1996)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50685
NCBI Official Full Name
caspase-2 isoform 2
NCBI Official Synonym Full Names
caspase 2, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP2
NCBI Official Synonym Symbols
ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
NCBI Protein Information
caspase-2
UniProt Protein Name
Caspase-2
UniProt Gene Name
CASP2
UniProt Synonym Gene Names
ICH1; NEDD2; CASP-2; NEDD-2
UniProt Entry Name
CASP2_HUMAN
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Product Notes
The CASP2 casp2 (Catalog #AAA285553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 198-226. The CASP2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CASP2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CASP2 casp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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