WB (Western Blot)
(Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Heart (Rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20 (bs-10442R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 47 kD)
Rabbit anti-Rat Caspase-1 p20 Polyclonal Antibody | anti-RHA3 antibody
Caspase-1 p20 Polyclonal Antibody
WB (Western Blot)
(Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Heart (Rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20 (bs-10442R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 47 kD)
WB (Western Blot)
(Sample: Lane 1: Heart (Mouse) Lysate at 40 ug Lane 2: Heart (Rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20 (bs-10442R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 47 kD)
FCM (Flow Cytometry)
(Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (bs-10442R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM (Flow Cytometry)
(Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (bs-10442R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM (Flow Cytometry)
(Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4 degree C) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (bs-10442R), Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC)
FCM (Flow Cytometry)
(Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4 degree C) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (bs-10442R), Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC)
WB (Western Blot)
(Dilution: Sample:large intestine (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(bs-10442R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD)
WB (Western Blot)
(Dilution: Sample:large intestine (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(bs-10442R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD)
WB (Western Blot)
(Sample:lung (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(bs-10442R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD)
WB (Western Blot)
(Sample:lung (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(bs-10442R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD)
IHC (Immunohistochemistry-Paraffin)
(Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated(bs-10442R) 1:500, overnight at 4?C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohistochemistry-Paraffin)
(Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated(bs-10442R) 1:500, overnight at 4?C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
NCBI and Uniprot Product Information
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Product Notes
The RHA3 rha3 (Catalog #AAA28810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-1 p20 Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-1 p20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the RHA3 rha3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-1 p20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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