FCM/FACS (Flow Cytometry)
(Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit CASPR antibody (bs-11128R) Dilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Rabbit CASPR Polyclonal Antibody | anti-CASPR antibody
CASPR Polyclonal Antibody
Gene Names
CNTNAP1; P190; CASPR; NRXN4; CNTNAP
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CASPR, Antibody; CASPR Polyclonal Antibody; Contactin-associated protein 1; Caspr; Caspr1; Neurexin IV; Neurexin-4; p190; CNTNAP1; CASPR; NRXN4; anti-CASPR antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-CASPR antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit CASPR antibody (bs-11128R) Dilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit CASPR antibody (bs-11128R) Dilution: 1 ug /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 ug /test.Protocol The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
ICC (Immunocytochemistry)
(Tissue/cell: human glioma cells(U251 cells);4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-CASPR Polyclonal Antibody, Unconjugated(bs-11128R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
ICC (Immunocytochemistry)
(Tissue/cell: human glioma cells(U251 cells);4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-CASPR Polyclonal Antibody, Unconjugated(bs-11128R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
156,267 Da
NCBI Official Full Name
contactin-associated protein 1
NCBI Official Synonym Full Names
contactin associated protein 1
NCBI Official Symbol
CNTNAP1
NCBI Official Synonym Symbols
P190; CASPR; NRXN4; CNTNAP
NCBI Protein Information
contactin-associated protein 1; caspr1; neurexin 4; neurexin-4; neurexin IV
UniProt Protein Name
Contactin-associated protein 1
UniProt Gene Name
CNTNAP1
UniProt Synonym Gene Names
CASPR; NRXN4; Caspr; Caspr1
UniProt Entry Name
CNTP1_HUMAN
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Product Notes
The CASPR cntnap1 (Catalog #AAA291308) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CASPR Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CASPR can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CASPR cntnap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CASPR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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