Rabbit Caveolin-3/CAV3 Polyclonal Antibody | anti-CAV3 antibody

Anti-Caveolin-3/CAV3 Antibody, Rabbit Polyclonal

Cat. Num. AAA258823

• Reactivity: Human, Rat, Bovin, Pig; (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Caveolin-3/CAV3, Antibody; Anti-Caveolin-3/CAV3 Antibody, Rabbit Polyclonal; caveolin 3; Anti-AI385751 Antibody; Anti-Cav-3 Antibody; Anti-M-cav Antibody; anti-CAV3 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Bovin, Pig; (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CAV3 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse Caveolin-3/CAV3
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Caveolin-3/CAV3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(CAV3 was immunoprecipitated using:Lane A:0.5 mg Rat heart Whole Cell Lysate4 uL anti-CAV3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CAV3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size :17 kDa)

IHC (Immunohistochemistry)

(Immunochemical staining of rat 3 in rat heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemisry)

(Immunochemical staining of Mouse CAV3 in Mouse skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of Mouse CAV3 in Mouse heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-Caveolin-3/CAV3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse heart tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa)

Related Product Information for anti-CAV3 antibody

Caveolinopathies are a heterogeneous family of genetic pathologies arising from alterations of the caveolin-3 gene (CAV3), encoding for the isoform specifically constituting muscle caveolae. Caveolin-3 (CAV3) is a principal structural protein of the caveolae membrane domains, which has been reported to participate in cell signaling as well as the maintenance of cell structure. Mutations in the CAV3 gene encoding caveolin-3 (Cav3), a scaffolding protein integral to caveolae in cardiomyocytes, have been associated with the congenital long-QT syndrome (LQT9). Mutations in caveolin-3 (CAV3) can lead to different clinical phenotypes affecting skeletal or cardiac muscles. Mutations in CAV3 lead to various neuromuscular phenotypes with partial overlap, including limb-girdle muscular dystrophy type 1C (LGMD1C), rippling muscle disease, distal myopathy, and isolated hyperCKemia.

References

Yang RB, et al. (2019) Overexpression of cav3 facilitates bone formation via the wnt signaling pathway in osteoporotic rats. Endocrine 63 (3): 639-650.Tyan L, et al. (2019) Long qt syndrome caveolin-3 mutations differentially modulate k(v) 4 and ca(v) 1.2 channels to contribute to action potential prolongation. J Physiol 597 (6): 1531-1551.
Stavusis J, et al. (2015) Cav3 gene sequence variations: National genome database and clinics. Acta Neurol Scand 132 (3): 185-190.
Scalco RS, et al. (2016) Cav3 mutations causing exercise intolerance, myalgia and rhabdomyolysis: Expanding the phenotypic spectrum of caveolinopathies. Neuromuscul Disord 26 (8): 504-510.