WB (Western Blot)
(Detection of human CCDC94 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC94 antibody (AAA213475 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human CCDC94 Polyclonal Antibody | anti-CCDC94 antibody
Rabbit anti-CCDC94 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CCDC94, Antibody; Rabbit anti-CCDC94 Antibody, Affinity Purified; CCDC94; YJU2 splicing factor homolog; coiled-coil domain containing 94; coiled-coil domain-containing protein 94; Coiled-coil domain-containing protein 94; anti-CCDC94 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CCDC94 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 273 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CCDC94 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC94 antibody (AAA213475 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human CCDC94 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC94 antibody (AAA213475 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CCDC94 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC94 antibody (AAA213475 lot 1) used for IP at 1 ug per reaction. CCDC94 was also immunoprecipitated by rabbit anti-CCDC94 antibody BL24066. For blotting immunoprecipitated CCDC94, AAA213475 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CCDC94 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC94 antibody (AAA213475 lot 1) used for IP at 1 ug per reaction. CCDC94 was also immunoprecipitated by rabbit anti-CCDC94 antibody BL24066. For blotting immunoprecipitated CCDC94, AAA213475 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-CCDC94 antibody
CCDC94 is part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. CCDC94 also plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates. CCDC94 may protect cells from TP53-dependent apoptosis upon dsDNA break damage through association with PRP19-CD5L complex. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BW85].
Product Categories/Family for anti-CCDC94 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,086 Da
NCBI Official Full Name
coiled-coil domain-containing protein 94
NCBI Official Synonym Full Names
coiled-coil domain containing 94
NCBI Official Symbol
CCDC94
NCBI Protein Information
coiled-coil domain-containing protein 94
UniProt Protein Name
Coiled-coil domain-containing protein 94
UniProt Gene Name
CCDC94
UniProt Entry Name
CCD94_HUMAN
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Product Notes
The CCDC94 ccdc94 (Catalog #AAA213475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CCDC94 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCDC94 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCDC94 ccdc94 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCDC94, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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