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WB (Western Blot)
(Western blot analysis of CCK4 (arrow) using rabbit polyclonal CCK4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human CCK4 (PTK7) Polyclonal Antibody | anti-PTK7 antibody
CCK4 (PTK7) Antibody (N-term)
Gene Names
PTK7; CCK4; CCK-4
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CCK4 (PTK7), Antibody; CCK4 (PTK7) Antibody (N-term); Inactive tyrosine-protein kinase 7; Colon carcinoma kinase 4; CCK-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; Tyrosine-protein kinase-like 7; PTK7; CCK4; anti-PTK7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CCK4 (PTK7) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human CCK4 (PTK7).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibodyis purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
21-52
Sequence Length
1,078
Applicable Applications for anti-PTK7 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both thenon-canonical (also known as the Wnt/planarcell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion,cell migration, cell polarity, proliferation, actincytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Cellular Location
Membrane; Single-pass type I membraneprotein Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at theleading edge of migrating cells.
Tissue Location
Highly expressed in lung, liver, pancreas,kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain,heart and skeletal muscle. Also expressed inerythroleukemia cells. But not expressed in colon.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thawcycles.
WB (Western Blot)
(Western blot analysis of CCK4 (arrow) using rabbit polyclonal CCK4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of CCK4 (arrow) using rabbit polyclonal CCK4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCI-H460 cells using CCK4 (PTK7) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCI-H460 cells using CCK4 (PTK7) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(All lanes : Anti-CCK4 (PTK7) Antibody (N-term)at 1:1000-1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Caco2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-CCK4 (PTK7) Antibody (N-term)at 1:1000-1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Caco2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PTK7 antibody
CCK4 may function as a cell adhesion molecule. Although it belongs to the insuline receptor subfamily of the Tyr protein kinases, it likely lacks the catalytic activity of a tyrosine kinase. It may be connected to the pathophysiology of colon carcinomas and/or may represent a tumor progression marker. This Type I membrane protein is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes, but weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle, and not in colon. It is also expressed in erythroleukemia cells.
Product Categories/Family for anti-PTK7 antibody
References
Zhang, H., et al., Nat. Biotechnol. 21(6):660-666 (2003).
Park, S.K., et al., J. Biochem. 119(2):235-239 (1996).
Mossie, K., et al., Oncogene 11(10):2179-2184 (1995).
Park, S.K., et al., J. Biochem. 119(2):235-239 (1996).
Mossie, K., et al., Oncogene 11(10):2179-2184 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
inactive tyrosine-protein kinase 7 isoform e
NCBI Official Synonym Full Names
protein tyrosine kinase 7 (inactive)
NCBI Official Symbol
PTK7
NCBI Official Synonym Symbols
CCK4; CCK-4
NCBI Protein Information
inactive tyrosine-protein kinase 7
UniProt Protein Name
Inactive tyrosine-protein kinase 7
UniProt Gene Name
PTK7
UniProt Synonym Gene Names
CCK4; CCK-4
UniProt Entry Name
PTK7_HUMAN
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Product Notes
The PTK7 ptk7 (Catalog #AAA285102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-52. The CCK4 (PTK7) Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCK4 (PTK7) can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PTK7 ptk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCK4 (PTK7), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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