WB (Western Blot)
(Western blot analysis of CCNB2 using 293 whole cell lysates)
Rabbit CCNB2 Polyclonal Antibody | anti-CCNB2 antibody
CCNB2 Antibody
Gene Names
CCNB2; HsT17299
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CCNB2, Antibody; CCNB2 Antibody; ccnb2; CCNB2_HUMAN; CycB2; Cyclin B2; G2 mitotic specific cyclin B2; G2/mitotic specific cyclin B2; G2/mitotic-specific cyclin-B2; HsT17299; MGC108931; MGC140694; anti-CCNB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CCNB2 antibody detects endogenous levels of total CCNB2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
398
Applicable Applications for anti-CCNB2 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CCNB2
Tissue Specificity
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of CCNB2 using 293 whole cell lysates)
WB (Western Blot)
(Western blot analysis of CCNB2 using 293 whole cell lysates)
IHC (Immunohistochemistry)
(AAA327777 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA327777 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CCNB2 antibody
Function: Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Subunit Structure: Interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.
Similarity: Belongs to the cyclin family. Cyclin AB subfamily.
Subunit Structure: Interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.
Similarity: Belongs to the cyclin family. Cyclin AB subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 45 kDa
Predicted: 46 kDa
Predicted: 46 kDa
NCBI Official Full Name
G2/mitotic-specific cyclin-B2
NCBI Official Synonym Full Names
cyclin B2
NCBI Official Symbol
CCNB2
NCBI Official Synonym Symbols
HsT17299
NCBI Protein Information
G2/mitotic-specific cyclin-B2
UniProt Protein Name
G2/mitotic-specific cyclin-B2
UniProt Gene Name
CCNB2
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Product Notes
The CCNB2 ccnb2 (Catalog #AAA327777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CCNB2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CCNB2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CCNB2 ccnb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCNB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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