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IF (Immunofluorescence)
(Immunofluorescence staining of His-MME in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human His-MME polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.)
Rabbit anti-Human CD10/Neprilysin Polyclonal Antibody | anti-MME antibody
Anti-CD10/Neprilysin Antibody, Rabbit Polyclonal
Gene Names
MME; NEP; SFE; CD10; CALLA
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CD10/Neprilysin, Antibody; Anti-CD10/Neprilysin Antibody, Rabbit Polyclonal; CD10/Neprilysin Antibody, Rabbit PAb, Antigen Affinity Purified; membrane metallo-endopeptidase; Anti-CALLA Antibody; Anti-CD10 Antibody; Anti-CMT2T Antibody; Anti-EPN Antibody; Anti-NEP Antibody; Anti-SCA43 Antibody; Anti-SFE Antibody; anti-MME antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CD10/Neprilysin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MME antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant Human CD10/Neprilysin Protein ( NP_000893.2; Tyr52-Trp750). CD10/Neprilysin specific IgG was purified by Human CD10/Neprilysin affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
IF (Immunofluorescence)
(Immunofluorescence staining of His-MME in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human His-MME polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of His-MME in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human His-MME polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.)
IHC (Immunohistochemisry)
(Immunochemical staining of human CD10 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human CD10 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human CD10 in human lymphoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human CD10 in human lymphoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-CD10 rabbit polyclonal antibody at 1:1000 dilution Lane A: Raji Whole Cell Lysate Lysates/proteins at 30ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa Observed band size:100 kDa)
WB (Western Blot)
(Anti-CD10 rabbit polyclonal antibody at 1:1000 dilution Lane A: Raji Whole Cell Lysate Lysates/proteins at 30ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa Observed band size:100 kDa)
Related Product Information for anti-MME antibody
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. The cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had the function to degrade some small secreted peptides such as the amyloid beta-peptide. It exists as a membrane-bound protein and has a high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.
References
Zol. H. e. al. (2007. C. molecule. 2006-huma. cel. differentiatio. molecules. . Immuno. Methods. 31. (1-2). 1-5.H. IC. e. al. (2009. GATA. an. th. T-cel. lineage. essentia. function. befor. an. afte. T-helper-2-cel. differentiation. Na. Re. Immunol. . (2). 125-35.Matesanz-Isabe. J. e. al. (2011. Ne. B-cel. C. molecules. Immunolog. Letters.13. (2). 104-12Dogan. e. al. (2000. CD1. an. BCL-. Expressio. i. Paraffi. Section. o. Norma. Lymphoi. Tissu. an. B-Cel. Lymphomas. America. Journa. o. Surgica. Pathology. 24(6). 846-52.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
750
NCBI Official Full Name
neprilysin
NCBI Official Synonym Full Names
membrane metallo-endopeptidase
NCBI Official Symbol
MME
NCBI Official Synonym Symbols
NEP; SFE; CD10; CALLA
NCBI Protein Information
neprilysin; enkephalinase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; common acute lymphocytic leukemia antigen; membrane metallo-endopeptidase
UniProt Protein Name
Neprilysin
UniProt Gene Name
MME
UniProt Synonym Gene Names
EPN; CALLA; NEP; Neutral endopeptidase; SFE
UniProt Entry Name
NEP_HUMAN
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Product Notes
The MME mme (Catalog #AAA259416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD10/Neprilysin Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD10/Neprilysin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MME mme for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD10/Neprilysin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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