FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-CD11c/Itgax antibody (AAA125543).Overlay histogram showing mouse spleen tissues stained with AAA125543 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11c/Itgax Antibody (AAA125543, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Mouse CD11c/Itgax Polyclonal Antibody | anti-Itgax antibody
Anti-CD11c/Itgax Antibody
Gene Names
Itgax; Cr4; N418; Cd11c; AI449405
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
CD11c/Itgax, Antibody; Anti-CD11c/Itgax Antibody; Integrin alpha-X; CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD11c; ITGAX; CD11C; integrin subunit alpha X; anti-Itgax antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for CD11c/Itgax detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-Itgax antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived mouse CD11c/Itgax recombinant protein (Position: F20-E332).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-CD11c/Itgax antibody (AAA125543).Overlay histogram showing mouse spleen tissues stained with AAA125543 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11c/Itgax Antibody (AAA125543, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-CD11c/Itgax antibody (AAA125543).Overlay histogram showing mouse spleen tissues stained with AAA125543 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11c/Itgax Antibody (AAA125543, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (AAA125543).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse spleen tissue lysatesLane 2: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CD11c/Itgax antigen affinity purified polyclonal antibody (Catalog # AAA125543) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD11c/Itgax at approximately 150KD. The expected band size for CD11c/Itgax is at 150KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (AAA125543).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse spleen tissue lysatesLane 2: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CD11c/Itgax antigen affinity purified polyclonal antibody (Catalog # AAA125543) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD11c/Itgax at approximately 150KD. The expected band size for CD11c/Itgax is at 150KD.)
Related Product Information for anti-Itgax antibody
CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a genethat encodes for CD11c. This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.
References
1. "Entrez Gene: ITGAX integrin, alpha X (complement component 3 receptor 4 subunit)".
2. Córbi AL, Lopéz-Rodríguez C (1997). "CD11c integrin gene promoter activity during myeloid differentiation. ". Leuk. Lymphoma. 25 (5-6): 415-25.
2. Córbi AL, Lopéz-Rodríguez C (1997). "CD11c integrin gene promoter activity during myeloid differentiation. ". Leuk. Lymphoma. 25 (5-6): 415-25.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
129,151 Da
NCBI Official Full Name
integrin alpha-X
NCBI Official Synonym Full Names
integrin alpha X
NCBI Official Symbol
Itgax
NCBI Official Synonym Symbols
Cr4; N418; Cd11c; AI449405
NCBI Protein Information
integrin alpha-X; CD11 antigen-like family member C; CD11C (p150) alpha polypeptide; complement receptor 4; integrin aX; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95
UniProt Protein Name
Integrin alpha-X
UniProt Gene Name
Itgax
UniProt Entry Name
ITAX_MOUSE
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Product Notes
The Itgax itgax (Catalog #AAA125543) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD11c/Itgax Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD11c/Itgax can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the Itgax itgax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11c/Itgax, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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