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WB (Western Blot) (Western Blot analysis of mouse brain cells using CD13 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)

Rabbit CD13 Polyclonal Antibody | anti-CD13 antibody

CD13 Rabbit Polyclonal Antibody

Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CD13; Polyclonal Antibody; CD13 Rabbit Polyclonal Antibody; ANPEP; APN; PEPN; Aminopeptidase N; AP-N; hAPN; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; BH6842; anti-CD13 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
CD13 Polyclonal Antibody detects endogenous levels of CD13 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD13 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC-p: 1:100-1:300
ELISA: 1/10000
Immunogen
Synthesized peptide derived from Aminopeptidase N at AA range: 881-930
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

WB (Western Blot)

(Western Blot analysis of mouse brain cells using CD13 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)

WB (Western Blot) (Western Blot analysis of mouse brain cells using CD13 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100)

WB (Western Blot)

(Western Blot analysis of mouse-liver mouse-heart mouse-kidney mouse-spleen mouse-brain mouse-heart using CD13 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000)

WB (Western Blot) (Western Blot analysis of mouse-liver mouse-heart mouse-kidney mouse-spleen mouse-brain mouse-heart using CD13 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Related Product Information for anti-CD13 antibody
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other Plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
290
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
109,540 Da
NCBI Official Full Name
aminopeptidase N
NCBI Official Synonym Full Names
alanyl (membrane) aminopeptidase
NCBI Official Symbol
ANPEP
NCBI Official Synonym Symbols
APN; CD13; LAP1; P150; PEPN; GP150
NCBI Protein Information
aminopeptidase N; AP-M; AP-N; hAPN; aminopeptidase M; alanyl aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13
UniProt Protein Name
Aminopeptidase N
UniProt Gene Name
ANPEP
UniProt Synonym Gene Names
APN; CD13; PEPN; AP-N; hAPN; AP-M
UniProt Entry Name
AMPN_HUMAN

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Product Notes

The CD13 anpep (Catalog #AAA30760) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD13 Rabbit Polyclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA). WB: 1/500-1/2000 IHC-p: 1:100-1:300 ELISA: 1/10000. Researchers should empirically determine the suitability of the CD13 anpep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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