WB (Western Blot)
(Immunoblot analysis of VE-cadherin and catenin expression in endothelial cells exposed to anti-Cad2 antibody. A, total cell lysates of untreated cells (lanes 1 and 4) and of cells treated with anti-Cad2 antibody for 2 h (lanes 2 and5) or 4 h (lanes 3 and6) were electrophoresed and transferred to nitrocellulose membranes before probing with anti-VE-Cad, anti-alpha-catenin, anti-beta-catenin, and anti-plakoglobin (plako) antibodies.Lanes 46 correspond to cells treated with cycloheximide for 4 h prior to the addition of anti-VE-Cad.B, shown are the results from the quantification of the amounts of VE-cadherin and catenins. The amounts of VE-cadherin (panel a), alpha-catenin (panel b), beta-catenin (panel c), and plakoglobin (panel d) were estimated, using image analysis software, from the ECL bands of the Western blots described for A. Shaded bars, without cycloheximide; white bars, with cycloheximide. The relative amounts of prote)
Rabbit anti-Human CD144 Polyclonal Antibody | anti-CD144 antibody
CD144 antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
CD144 antibody was purified by affinity chromatography.
Synonyms
CD144, Antibody; CD144 antibody; Polyclonal CD144; Anti-CD144; CD 144; CD-144; VE-Cadherin; CD144; anti-CD144 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human
Purity/Purification
CD144 antibody was purified by affinity chromatography.
Form/Format
Purified IgG supplied in PBS.
Applicable Applications for anti-CD144 antibody
WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
Biological Significance
VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell
Cross-Reactivity
Bovine, mouse, rat
Immunogen
CD144 antibody was raised in rabbit using recombinant protein fragments corresponding to amino acids 1-258 of VE-cadherin as the immunogen.
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles
WB (Western Blot)
(Immunoblot analysis of VE-cadherin and catenin expression in endothelial cells exposed to anti-Cad2 antibody. A, total cell lysates of untreated cells (lanes 1 and 4) and of cells treated with anti-Cad2 antibody for 2 h (lanes 2 and5) or 4 h (lanes 3 and6) were electrophoresed and transferred to nitrocellulose membranes before probing with anti-VE-Cad, anti-alpha-catenin, anti-beta-catenin, and anti-plakoglobin (plako) antibodies.Lanes 46 correspond to cells treated with cycloheximide for 4 h prior to the addition of anti-VE-Cad.B, shown are the results from the quantification of the amounts of VE-cadherin and catenins. The amounts of VE-cadherin (panel a), alpha-catenin (panel b), beta-catenin (panel c), and plakoglobin (panel d) were estimated, using image analysis software, from the ECL bands of the Western blots described for A. Shaded bars, without cycloheximide; white bars, with cycloheximide. The relative amounts of prote)
WB (Western Blot)
(Immunoblot analysis of VE-cadherin and catenin expression in endothelial cells exposed to anti-Cad2 antibody. A, total cell lysates of untreated cells (lanes 1 and 4) and of cells treated with anti-Cad2 antibody for 2 h (lanes 2 and5) or 4 h (lanes 3 and6) were electrophoresed and transferred to nitrocellulose membranes before probing with anti-VE-Cad, anti-alpha-catenin, anti-beta-catenin, and anti-plakoglobin (plako) antibodies.Lanes 46 correspond to cells treated with cycloheximide for 4 h prior to the addition of anti-VE-Cad.B, shown are the results from the quantification of the amounts of VE-cadherin and catenins. The amounts of VE-cadherin (panel a), alpha-catenin (panel b), beta-catenin (panel c), and plakoglobin (panel d) were estimated, using image analysis software, from the ECL bands of the Western blots described for A. Shaded bars, without cycloheximide; white bars, with cycloheximide. The relative amounts of prote)
FCM/FACS (Flow Cytometry)
(Recognition of native VE-cadherin by anti-VE-Cad. Specificity of the anti-fragment antibody anti-VE-Cad was examined by flow cytometry using VE-cadherin-expressing CHO cells(A) (hatched surface, VE-cadherin transfectant; open surface, CHO control), by immunofluorescence staining of fixed confluent endothelial cell monolayers (B), or by Western blot using endothelial cell lysates (C).)
FCM/FACS (Flow Cytometry)
(Recognition of native VE-cadherin by anti-VE-Cad. Specificity of the anti-fragment antibody anti-VE-Cad was examined by flow cytometry using VE-cadherin-expressing CHO cells(A) (hatched surface, VE-cadherin transfectant; open surface, CHO control), by immunofluorescence staining of fixed confluent endothelial cell monolayers (B), or by Western blot using endothelial cell lysates (C).)
Related Product Information for anti-CD144 antibody
Rabbit polyclonal CD144 antibody
Product Categories/Family for anti-CD144 antibody
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Product Notes
The CD144 (Catalog #AAA74966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD144 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD144 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD144 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD144, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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