Standard Curve (Sample)
Mouse CD144 ELISA Kit | CDH5 elisa kit
Mouse CD144 Cadherin ELISA Kit
Gene Names
Cdh5; 7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
Reactivity
Mouse
Applications
ELISA
Synonyms
CD144; N/A; Mouse CD144 Cadherin ELISA Kit; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CDH5 elisa kit
Reactivity
Mouse
Specificity
The Mouse CD144 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD144 proteins within the range of 312 pg/ml - 20000 pg/ml.
Sequence Length
784
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Applicable Applications for CDH5 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CDH5 elisa kit
Principle of the Assay||The Mouse CD144 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD144 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD144 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD144 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD144 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD144 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of theorganization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.
Background/Introduction: Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of theorganization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
87,903 Da
NCBI Official Full Name
Cadherin-5
NCBI Official Synonym Full Names
cadherin 5
NCBI Official Symbol
Cdh5
NCBI Official Synonym Symbols
7B4; Vec; VECD; Cd144; VEcad; VE-Cad; AA408225
NCBI Protein Information
cadherin-5
UniProt Protein Name
Cadherin-5
UniProt Gene Name
Cdh5
UniProt Synonym Gene Names
VE-cadherin
UniProt Entry Name
CADH5_MOUSE
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Product Notes
The Mouse CDH5 cdh5 (Catalog #AAA261780) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261780 ELISA Kit recognizes Mouse CDH5. AAA Biotech's CD144 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the CDH5 cdh5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD144, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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