WB (Western Blot)
(Detection of human CD168/RHAMM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD168/RHAMM antibody AAA212937 (lot AAA212937-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human CD168/RHAMM Polyclonal Antibody | anti-CD168/RHAMM antibody
Rabbit anti-CD168/RHAMM Antibody, Affinity Purified
Gene Names
HMMR; CD168; IHABP; RHAMM
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CD168/RHAMM, Antibody; Rabbit anti-CD168/RHAMM Antibody, Affinity Purified; CD168; receptor for hyaluronan-mediated motility; intracellular hyaluronic acid-binding protein; hyaluronan mediated motility receptor; CD antigen CD168; RHAMM; IHABP; Hyaluronan mediated motility receptor; anti-CD168/RHAMM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CD168/RHAMM antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD168/RHAMM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD168/RHAMM antibody AAA212937 (lot AAA212937-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human CD168/RHAMM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CD168/RHAMM antibody AAA212937 (lot AAA212937-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CD168/RHAMM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD168/RHAMM antibody AAA212937 (lot AAA212937-1) used for IP at 6 ug per reaction. CD168/RHAMM was also immunoprecipitated by rabbit anti-CD168/RHAMM antibody BL18745. For blotting immunoprecipitated CD168/RHAMM, AAA212937 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CD168/RHAMM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CD168/RHAMM antibody AAA212937 (lot AAA212937-1) used for IP at 6 ug per reaction. CD168/RHAMM was also immunoprecipitated by rabbit anti-CD168/RHAMM antibody BL18745. For blotting immunoprecipitated CD168/RHAMM, AAA212937 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-CD168/RHAMM antibody
CD168 /RHAMM (Receptor for hyaluronan-mediated motility) is involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. it may also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75330].
Product Categories/Family for anti-CD168/RHAMM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,100 Da
NCBI Official Full Name
hyaluronan mediated motility receptor isoform c
NCBI Official Synonym Full Names
hyaluronan-mediated motility receptor (RHAMM)
NCBI Official Symbol
HMMR
NCBI Official Synonym Symbols
CD168; IHABP; RHAMM
NCBI Protein Information
hyaluronan mediated motility receptor; receptor for hyaluronan-mediated motility; intracellular hyaluronic acid-binding protein
UniProt Protein Name
Hyaluronan mediated motility receptor
UniProt Gene Name
HMMR
UniProt Synonym Gene Names
IHABP; RHAMM
UniProt Entry Name
HMMR_HUMAN
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Product Notes
The CD168/RHAMM hmmr (Catalog #AAA212937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD168/RHAMM Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD168/RHAMM can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD168/RHAMM hmmr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD168/RHAMM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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