Rabbit anti-Human CD19 Polyclonal Antibody | anti-CD19 antibody

Anti-CD19 Antibody, Rabbit Polyclonal

Cat. Num. AAA258899

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: CD19, Antibody; Anti-CD19 Antibody, Rabbit Polyclonal; CD19 molecule; Anti-B4 Antibody; Anti-CVID3 Antibody; anti-CD19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD19
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD19 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human CD19 Protein
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD19 (rh CD19). CD19 specific IgG was purified by Human CD19 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !

IHC (Immunohistochemisry)

(Immunochemical staining of human CD19 in human spleen with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human CD19 in human tonsil with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-CD19 rabbit polyclonal antibody at 1:500 dilutionLane A: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:61 kDa)

Related Product Information for anti-CD19 antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in the threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell co-receptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.